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Culture of Rodent Cortical, Hippocampal, and Striatal Neurons

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Neurotrophic Factors

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1727))

Abstract

Neurons cultured from rodent central nervous system tissues represent important tools in the study of neurodegenerative disease mechanisms and neuroregenerative processes, including the survival- and axon growth-promoting properties of neurotrophic factors. This chapter presents a detailed protocol for the preparation of rat and mouse cortical, hippocampal, and striatal neuron cell cultures, using either embryonic or postnatal tissue with enzymatic digestion.

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Authors and Affiliations

  1. Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Padua, Italy

    Laura Facci & Stephen D. Skaper

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  1. Laura Facci
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  2. Stephen D. Skaper
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Correspondence to Stephen D. Skaper .

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Editors and Affiliations

  1. Department of Pharmaceutical and Pharmacological Sciences, University of Padova, Padova, Italy

    Stephen D. Skaper

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Facci, L., Skaper, S.D. (2018). Culture of Rodent Cortical, Hippocampal, and Striatal Neurons. In: Skaper, S. (eds) Neurotrophic Factors. Methods in Molecular Biology, vol 1727. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7571-6_3

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  • DOI: https://doi.org/10.1007/978-1-4939-7571-6_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7570-9

  • Online ISBN: 978-1-4939-7571-6

  • eBook Packages: Springer Protocols

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