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Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer

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Chaperones

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1709))

Abstract

Hsp60 (also called Cpn60) is a chaperonin with essential functions for cell physiology and survival. Additionally, its involvement in the pathogenesis of a variety of diseases (e.g., some autoimmune disorders and cancer) is becoming evident with new research. For example, the distribution and levels of Hsp60 in cells and tissues have been found altered in many pathologic conditions, and the significance of these alterations is being investigated in a number of laboratories. The aim of this ongoing research is to determine the meaning of these Hsp60 alterations with regard to pathogenetic mechanisms, diagnosis, classification of lesions, and assessing prognosis and response to treatment.

Hsp60 occurs in the mitochondria, i.e., its typical residence according to classic knowledge, and also in other locales, such as the cytosol, the cell membrane, the intercellular space, and biological fluids (e.g., blood and cerebrospinal fluid). Detection and quantitative determinations in all these locations are becoming essential components of laboratory pathology in clinics and research. Consequently, immunohistochemistry targeting Hsp60 is also becoming essential for pathologists and researchers interested in disorders involving this chaperonin.

In this chapter, we summarize some recent discoveries on the participation of Hsp60 in the pathogenesis of human diseases, and describe in detail how to perform immunohistochemical reactions for detecting the chaperonin, determining its location, and measuring its quantitative levels.

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Acknowledgments

A.J.L.M. and E.C. de M. were partially supported by IMET; A.J.L.M. and F.C. were partially supported by IEMEST. This work was done under the umbrella of the agreement between the Euro-Mediterranean Institute of Science and Technology (IEMEST; Italy) and the Institute of Marine and Environmental Technology (IMET; USA) signed March 2012 (this is IMET contribution number IMET 17-195). In this work were used instruments provided by the Euro-Mediterranean Institute of Science and Technology, and funded by the Italian National Operational Programme for Research and Competitiveness 2007–2013 grant (Project code: PONa3_00210, European Regional Development Fund).

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Authors and Affiliations

  1. Human Anatomy Section, Department of Experimental Biomedicine and Clinical Neurosciences, University of Palermo, Palermo, Italy

    Francesco Cappello, Francesca Rappa & Giovanni Zummo

  2. Euro-Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy

    Francesco Cappello, Everly Conway de Macario, Francesca Rappa & Alberto J. L. Macario

  3. Department of Microbiology and Immunology, School of Medicine, University of Maryland at Baltimore, and IMET; Columbus Center, 701 East Pratt Street, Baltimore, MD, 21202, USA

    Everly Conway de Macario & Alberto J. L. Macario

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  1. Francesco Cappello
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  2. Everly Conway de Macario
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  3. Francesca Rappa
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  4. Giovanni Zummo
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  5. Alberto J. L. Macario
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Correspondence to Alberto J. L. Macario .

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Editors and Affiliations

  1. Department of Radiation, Oncology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, Massachusetts, USA

    Stuart K. Calderwood

  2. Department of Urology, Weis Center for Research, Geisinger Clinic, Danville, Pennsylvania, USA

    Thomas L. Prince

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Cappello, F., Conway de Macario, E., Rappa, F., Zummo, G., Macario, A.J.L. (2018). Immunohistochemistry of Human Hsp60 in Health and Disease: From Autoimmunity to Cancer. In: Calderwood, S., Prince, T. (eds) Chaperones. Methods in Molecular Biology, vol 1709. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7477-1_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7477-1_21

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