Abstract
Over the last decades several mouse models of human hemoglobin disorders have been and continue to be generated. This chapter aims at describing various approaches to evaluate the global red blood cell properties in mouse models of human hemoglobin disorders, in particular thalassemia and sickle cell disease. Analysis of erythroid parameters provides insights into the RBC physiologic or pathophysiologic status. Mice expressing both murine and human globin genes can be investigated using adapted protocols provided herein.
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Acknowledgment
This work was supported by Canadian Blood Services and Canadian Institute for Health Research grants to M.T.
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Trudel, M., Sedzro, JC. (2018). Approaches for Analysis of Erythroid Cell Parameters and Hemoglobinopathies in Mouse Models. In: Lloyd, J. (eds) Erythropoiesis. Methods in Molecular Biology, vol 1698. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7428-3_6
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DOI: https://doi.org/10.1007/978-1-4939-7428-3_6
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