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Optogenetics: A Roadmap

  • Book
  • © 2018

Overview

Editors:
  1. Albrecht Stroh

    1. Focus Program Translational Neurosciences and Institute for Microscopic Anatomy and Neurobiology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany

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  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Neuromethods (NM, volume 133)

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Table of contents (14 protocols)

  1. Front Matter

    Pages i-xii
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  2. A Hitchhiker’s Guide to the Selection of Viral Vectors for Optogenetic Studies

    • Kimberly R. Thompson, Chris Towne
    Pages 1-23

  3. Cell Type-Specific Targeting Strategies for Optogenetics

    • Ofer Yizhar, Antoine Adamantidis
    Pages 25-42

  4. Molecular Engineering of Channelrhodopsins for Enhanced Control over the Nervous System

    • André Berndt
    Pages 43-62

  5. Optogenetic Control of Intracellular Signaling: Class II Opsins

    • Erik Ellwardt, Raag D. Airan
    Pages 63-73

  6. Optogenetics in Stem Cell Research: Focus on the Central Nervous System

    • Johannes Boltze, Albrecht Stroh
    Pages 75-87

  7. Optogenetic Applications in the Nematode Caenorhabditis elegans

    • Katharina Elisabeth Fischer, Nathalie Alexandra Vladis, Karl Emanuel Busch
    Pages 89-116

  8. Optogenetic Interpellation of Behavior Employing Unrestrained Zebrafish Larvae

    • Soojin Ryu, Rodrigo J. De Marco
    Pages 117-131

  9. Combining Optogenetics with MEA, Depth-Resolved LFPs and Assessing the Scope of Optogenetic Network Modulation

    • Jenq-Wei Yang, Pierre-Hugues Prouvot, Albrecht Stroh, Heiko J. Luhmann
    Pages 133-152

  10. Concepts of All-Optical Physiology

    • Jan Doering, Ting Fu, Isabelle Arnoux, Albrecht Stroh
    Pages 153-174

  11. Two-Photon Optogenetics by Computer-Generated Holography

    • Eirini Papagiakoumou, Emiliano Ronzitti, I-Wen Chen, Marta Gajowa, Alexis Picot, Valentina Emiliani
    Pages 175-197

  12. Optophysiology and Behavior in Rodents and Nonhuman Primates

    • Golan Karvat, Ilka Diester
    Pages 199-217

  13. Employing Optogenetics in Memory Research

    • Limor Regev, Inbal Goshen
    Pages 219-256

  14. Towards Opto-Magnetic Physiology: Concepts and Pitfalls of ofMRI

    • Miriam Schwalm, Eduardo Rosales Jubal, Albrecht Stroh
    Pages 257-276

  15. Optogenetics: Lighting a Path from the Laboratory to the Clinic

    • Hannah K. Kim, Allyson L. Alexander, Ivan Soltesz
    Pages 277-300

  16. Back Matter

    Pages 301-302
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Keywords

About this book

This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience. This book shows readers how to identify the critical aspects of each methodological step, and how to determine the necessary level of complexity to address the particular research question. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Comprehensive and cutting-edge, Optogenetics: A Roadmap is a valuable guide for both the optogenetics novices and the experts.

Editors and Affiliations

  • Focus Program Translational Neurosciences and Institute for Microscopic Anatomy and Neurobiology, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany

    Albrecht Stroh

Bibliographic Information

  • Book Title: Optogenetics: A Roadmap

  • Editors: Albrecht Stroh

  • Series Title: Neuromethods

  • DOI: https://doi.org/10.1007/978-1-4939-7417-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2018

  • Hardcover ISBN: 978-1-4939-7415-3Published: 25 October 2017

  • Softcover ISBN: 978-1-4939-8479-4Published: 23 May 2018

  • eBook ISBN: 978-1-4939-7417-7Published: 24 October 2017

  • Series ISSN: 0893-2336

  • Series E-ISSN: 1940-6045

  • Edition Number: 1

  • Number of Pages: XII, 302

  • Number of Illustrations: 3 b/w illustrations, 63 illustrations in colour

  • Topics: Neurosciences

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