Abstract
Here we describe a protocol for the determination of polyamines using capillary electrophoresis with ultraviolet absorbance detection. Aliphatic polyamines were derivatized with salicylaldehyde-5-sulfonate (SAS) which formed Schiff base with amino groups, resulting in anionic derivatives. The derivatization of polyamines, including putrescine (PUT), cadaverine (CAD), spermidine (SPD), and spermine (SPM), was conducted in 10 mM HEPES buffer with pH 7.8 containing 5 mM SAS in a mixed solvent of water and ethanol. The SAS derivatives were separated using a background electrolyte composed of 10 mM phosphate buffer with pH 7.8. Calibration curves were linear over a concentration range of 20–200 μM for CAD, PUT, SPD, and SPM, and the limits of detection (LOD) were several μM for all polyamines. Furthermore, solid phase extraction was coupled with the CE method to improve the LOD to sub-μM levels, and the calibration curves were linear over a concentration range of 1–20 μM for CAD, PUT, and SPD.
References
Jänne J, Pösö H, Raina A (1978) Polyamines in rapid growth and cancer. Biochim Biophys Acta 473:241–293
Luk GD, Casero RA (1987) Polyamines in normal and cancer cells. Adv Enzym Regul 26:91–105
Busto O, Valero Y, Guasch J, Borrull F (1994) Solid phase extraction applied to the determination of biogenic amines in wines by HPLC. Chromatographia 38:571–578
Busto O, Mestres M, Guasch J, Borrull F (1995) Determination of biogenic amines in wine after clean-up by solid-phase extraction. Chromatographia 40:404–410
Saccani G, Tanzi E, Pastore P, Cavalli S, Reyd M (2005) Determination of biogenic amines in fresh and processed meat by suppressed ion chromatography-mass spectrometry using a cation-exchange column. J Chromatogr A 1082:43–50
Mattusch J, Huhn G, Wennrich R (1995) Sensitive laser induced fluorescence detection of polyamine-fluoresceinisothiocyanate-derivatives after capillary zone electrophoretic separation. Fresenius J Anal Chem 351:732–738
Legaz ME, Vicente C, Pedrosa MM (1998) Separation of tosylated polyamines by high-performance capillary zone electrophoresis. J Chromatogr A 823:511–521
Paproski RE, Roy KI, Lucy CA (2002) Selective fluorometric detection of polyamines using micellar electrokinetic chromatography with laser-induced fluorescence detection. J Chromatogr A 946:265–273
Liu G, Chen J, Ma Y (2004) Simultaneous determination of catecholamines and polyamines in PC-12 cell extracts by micellar electrokinetic capillary chromatography with ultraviolet absorbance detection. J Chromatogr B805:281–288
Zhang L-Y, Tang X-C, Sun M-X (2005) Simultaneous determination of histamine and polyamines by capillary zone electrophoresis with 4-fluor-7-nitro-2,1,3-benzoxadiazole derivatization and fluorescence detection. J Chromatogr B 820:211–219
Fu N-N, Zhang H-S, Ma M, Wang H (2007) Quantification of polyamines in human erythrocytes using a new near-infrared cyanine 1-(epsilon-succinimidyl-hexanoate)-1′-methyl-3,3,3′,3′-tetramethyl-indocarbocyanine-5,5′-disulfonate potassium with CE-LIF detection. Electrophoresis 28:822–829
Driouich R, Takayanagi T, Oshima M, Motomizu S (2001) Separation and determination of n-alkylamines and histamine by capillary zone electrophoresis using salicylaldehyde-5-sulfonate as a derivatizing reagent. J Chromatogr A 934:95–103
Driouich R, Takayanagi T, Oshima M, Motomizu S (2003) Investigation of salicylaldehyde-5-sulfonate as a precolumn derivatizing agent for the determination of n-alkane diamines, lysine, diaminopimelic acid, and isoniazid by capillary zone electrophoresis. J Pharm Biomed Anal 30:1523–1530
Inoue G, Kaneta T, Takayanagi T, Kakehi J, Motose H, Takahashi T (2013) Determination of polyamines in Arabidopsis Thaliana by capillary electrophoresis using salicylaldehyde-5-sulfonate as a derivatizing reagent. Anal Methods 5:2854–2859
Berry KJ, Moya F, Murray KS, van den Bergen AMB, West BO (1982)Water-solublecobalt(II) complexes ofNN'-substitutedbis(salicylaldimine-5-sulphonic acids).Oxygen-carryingproperties and conversion into cobalt(III) organometallic compounds. J Chem Soc Dalton Trans 1982:109–116
Baugh LS, Sissano JA (2002) Polymerization of methyl methacrylate and other polar monomers with alkylaluminum initiators bearing bidentate and tridentate N- and O-donor ligands. J Polym Sci Part A Polym Chem 40:1633–1651
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Kaneta, T. (2018). Determination of Polyamines by Capillary Electrophoresis Using Salicylaldehyde-5-Sulfonate as a Derivatizing Reagent. In: Alcázar, R., Tiburcio, A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7398-9_5
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DOI: https://doi.org/10.1007/978-1-4939-7398-9_5
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