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Pentamine as a Substrate for Measuring Spermine Oxidase Activity

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Polyamines

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1694))

Abstract

A method for determining spermine oxidase activity is described using the pentamine 1,16-diamino-4,8,13-triazahexadecane (3343) as the substrate, coupled with o-phthalaldehyde-post-label ion-exchange HPLC. The synthesis of 3343 is also described.

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References

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Acknowledgements

The authors would like to thank Dr. Keijiro Samejima for his help with this manuscript.

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Authors and Affiliations

  1. Laboratory of Bioorganic Chemistry, Department of Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, Keyaki-dai, Saitama, 350-0295, Japan

    Koichi Takao & Yoshiaki Sugita

Authors
  1. Koichi Takao
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  2. Yoshiaki Sugita
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Corresponding author

Correspondence to Koichi Takao .

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Editors and Affiliations

  1. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Rubén Alcázar

  2. Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Section of Plant Physiology, University of Barcelona, Spain

    Antonio F. Tiburcio

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Takao, K., Sugita, Y. (2018). Pentamine as a Substrate for Measuring Spermine Oxidase Activity. In: Alcázar, R., Tiburcio, A. (eds) Polyamines. Methods in Molecular Biology, vol 1694. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7398-9_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7398-9_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7397-2

  • Online ISBN: 978-1-4939-7398-9

  • eBook Packages: Springer Protocols

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