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Allergenicity Assessment of Transgenic Wheat Lines In Silico

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Wheat Biotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1679))

Abstract

Agriculture biotechnology is a promising tool for developing varieties with enhanced quality and quantity. Transgenic proteins expressed by genetically modified (GM) food crops improve crop characteristics like nutritional value, taste, and texture, and endow plants with resistance against fungus, pests, and insects. Despite such potential benefits, there are concerns regarding possible adverse effects of GM crops on human health, animals and the environment. Among the proposed guidelines for GM food safety testing—the weight-of-evidence approach proposed by the Codex Alimentarius Commission (ALINORM 03/34A) is the most recent. Till date, several transgenic wheat lines have been developed and research is underway for further improvement. However, GM wheat is not being grown or consumed in any part of the world. In the present study, in silico tools were employed for safety testing of eight transgenes used for the development of transgenic wheat lines. Among the genes studied, none of them shared sequence homology with the reported allergens and may be safe for use in genetic engineering. In conclusion, gene selection for developing transgenic wheat lines should be done with utmost care to ensure its safety for feed and fodder.

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Author information

Authors and Affiliations

  1. Allergy and Immunology Section, CSIR—Institute of Genomics and Integrative Biology, Room 509, Delhi University Campus, Mall Road, Delhi, 110007, India

    Ankita Mishra & Naveen Arora

Authors
  1. Ankita Mishra
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  2. Naveen Arora
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Corresponding author

Correspondence to Naveen Arora .

Editor information

Editors and Affiliations

  1. Plant Molecular Biology and Biotechnology Laboratory, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia

    Prem L. Bhalla

  2. Plant Molecular Biology and Biotechnology Laboratory, School of Agriculture and Food, Faculty of Veterinary and Agricultural Sciences, The University of Melbourne, Parkville, Victoria, Australia

    Mohan B. Singh

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Mishra, A., Arora, N. (2017). Allergenicity Assessment of Transgenic Wheat Lines In Silico. In: Bhalla, P., Singh, M. (eds) Wheat Biotechnology. Methods in Molecular Biology, vol 1679. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7337-8_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7337-8_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7335-4

  • Online ISBN: 978-1-4939-7337-8

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