Abstract
Chromatin immunoprecipitation (ChIP) is a widely used and very powerful procedure to identify the proteins that are associated with the DNA to regulate developmental processes. These proteins can be transcription factors, or specific histone variants and modified histones, which are all crucial for gene regulation. In order to obtain reliable results, ChIP must be carried out under highly reproducible conditions. Here, we describe a simple and fast ChIP protocol adapted for Arabidopsis seedlings, which can serve as a basis for other species, organs or more sophisticated procedures, such as the sequential ChIP. We also provide user-oriented troubleshooting to increase the chances of successful applications.
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Acknowledgments
Z.V. and S.M. were recipients of Fellowships BES-2010-037158 (MINECO; Spain) and SFRH/BD/105550/2014 (FCT, Portugal), respectively. This work has been supported by grants BFU2012-34821 (MINECO), BIO2013-50098-EXP (MINECO) and BFU2015-68396-R (MINECO-FEDER), and by an institutional grant from Fundación Ramón Areces to the Centro de Biología Molecular Severo Ochoa.
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Desvoyes, B., Vergara, Z., Sequeira-Mendes, J., Madeira, S., Gutierrez, C. (2018). A Rapid and Efficient ChIP Protocol to Profile Chromatin Binding Proteins and Epigenetic Modifications in Arabidopsis. In: Bemer, M., Baroux, C. (eds) Plant Chromatin Dynamics. Methods in Molecular Biology, vol 1675. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7318-7_5
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DOI: https://doi.org/10.1007/978-1-4939-7318-7_5
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Publisher Name: Humana Press, New York, NY
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