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Cryoinjury Model for Tissue Injury and Repair in Bioengineered Human Striated Muscle

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Skeletal Muscle Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1668))

Abstract

Regenerative medicine aims to replace injured tissues to restore normal physiological function. One possibility for achieving this goal is to activate or enhance endogenous regenerative pathways. Therefore, human tissue regeneration models may be useful tools for the discovery and development of novel regenerative therapeutics. In this chapter, we describe methods for the generation of three-dimensional bioengineered striated muscle in vitro and a cryoinjury model that can be applied to these tissues. This technique enables mechanistic in vitro analysis of the endogenous regenerative response of human striated muscle to injury, which is not possible using other in vivo approaches.

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Authors and Affiliations

  1. Cardiac Regeneration Laboratory, School of Biomedical Sciences, The University of Queensland, St Lucia, QLD, 4072, Australia

    Richard J. Mills, Holly K. Voges, Enzo R. Porrello Ph.D. & James E. Hudson Ph.D.

Authors
  1. Richard J. Mills
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  2. Holly K. Voges
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  3. Enzo R. Porrello Ph.D.
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  4. James E. Hudson Ph.D.
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Corresponding author

Correspondence to James E. Hudson Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Physiology, The University of Melbourne, Parkville, Victoria, Australia

    James G. Ryall

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Mills, R.J., Voges, H.K., Porrello, E.R., Hudson, J.E. (2017). Cryoinjury Model for Tissue Injury and Repair in Bioengineered Human Striated Muscle. In: Ryall, J. (eds) Skeletal Muscle Development. Methods in Molecular Biology, vol 1668. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7283-8_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7283-8_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7282-1

  • Online ISBN: 978-1-4939-7283-8

  • eBook Packages: Springer Protocols

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