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Protein Arrays II: Antigen Arrays

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Functional Genomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1654))

Abstract

Antigen arrays are fabricated using various antigens such as DNA, histones, synthetic peptides, recombinant proteins, or cell extracts to detect autoantibodies in autoimmune diseases, alloantibodies in transplantation, drug-induced antibodies or cancer-induced antibodies in blood or cell culture supernatant. In this protocol, we will provide a step-by-step executable procedure to perform antigen arrays, including antigen preparation and printing, blocking, sample loading, array detection, imaging, and data analysis.

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References

  1. Prechl J, Papp K, Erdei A (2010) Antigen microarrays: descriptive chemistry or functional immunomics? Trends Immunol 31(4):133–137

    Article  CAS  PubMed  Google Scholar 

  2. Li QZ, Xie C, Wu T, Mackay M, Aranow C, Putterman C, Mohan C (2005) Identification of autoantibody clusters that best predict lupus disease activity using glomerular proteome arrays. J Clin Invest 115(12):3428–3439. doi:10.1172/JCI23587

    Article  PubMed  Google Scholar 

  3. Ramachandran N, Raphael JV, Hainsworth E, Demirkan G, Fuentes MG, Rolfs A, Hu Y, LaBaer J (2008) Next-generation high-density self-assembling functional protein arrays. Nat Methods 5(6):535–538

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Tabakman SM, Lau L, Robinson JT, Price J, Sherlock SP, Wang H, Zhang B, Chen Z, Tangsombatvisit S, Jarrell JA, Utz PJ, Dai H (2011) Plasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range. Nat Commun 2:466. doi:10.1038/ncomms1477

    Article  PubMed  PubMed Central  Google Scholar 

  5. Zhang B, Kumar RB, Dai H, Feldman BJ (2014) A plasmonic chip for biomarker discovery and diagnosis of type 1 diabetes. Nat Med 20(8):948–953. doi:10.1038/nm.3619

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Papp K, Prechl J (2012) The use of antigen microarrays in antibody profiling. Methods Mol Biol 815:175–185

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

This work was partly supported by a grant from the Lupus Research Institute to T.W. and a startup fund from the University of Houston to T.W.

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Authors and Affiliations

  1. Department of Biomedical Engineering, University of Houston, 3605 Cullen Blvd, Houston, TX, 77204-5060, USA

    Yulin Yuan, Hongting Wang, Zuan-Tao Lin, Xia Hong, Mikala Heon & Tianfu Wu Ph.D.

  2. Department of Clinical Laboratory, The People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China

    Yulin Yuan

  3. National Pharmacology Laboratory of Chinese Medicine, College of Basic Medical Sciences, Wannan Medical College, Wuhu, China

    Hongting Wang

  4. Department of Nursing, Fujian Health College, Fuzhou, Fujian, China

    Xia Hong

Authors
  1. Yulin Yuan
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  2. Hongting Wang
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  3. Zuan-Tao Lin
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  4. Xia Hong
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  5. Mikala Heon
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  6. Tianfu Wu Ph.D.
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Corresponding author

Correspondence to Tianfu Wu Ph.D. .

Editor information

Editors and Affiliations

  1. Chair of Biochemistry and Molecular Medicine, Division of Functional Genomics, Faculty of Health, School of Medicine, Center for Biochemical Research and Education ZBAF, Witten/Herdecke University, Witten, Germany

    Michael Kaufmann

  2. Chair of Biochemistry and Molecular Medicine, Division of Functional Genomics, Faculty of Health, School of Medicine, Center for Biochemical Research and Education ZBAF, Witten/Herdecke University, Witten, Germany

    Claudia Klinger

  3. Chair of Biochemistry and Molecular Medicine, Division of Functional Genomics, Faculty of Health, School of Medicine, Center for Biochemical Research and Education ZBAF, Witten/Herdecke University, Witten, Germany

    Andreas Savelsbergh

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Yuan, Y., Wang, H., Lin, ZT., Hong, X., Heon, M., Wu, T. (2017). Protein Arrays II: Antigen Arrays. In: Kaufmann, M., Klinger, C., Savelsbergh, A. (eds) Functional Genomics. Methods in Molecular Biology, vol 1654. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7231-9_20

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  • DOI: https://doi.org/10.1007/978-1-4939-7231-9_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7230-2

  • Online ISBN: 978-1-4939-7231-9

  • eBook Packages: Springer Protocols

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