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Isolation and Characterization of Chicken Primordial Germ Cells and Their Application in Transgenesis

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Avian and Reptilian Developmental Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1650))

Abstract

Primordial germ cells (PGCs), precursors of functional gametes, offer great promise for the use of genetic resources and transgenesis in chickens. PGCs can be isolated from the developing embryo at diverse early stages and are subsequently expandable in vitro. In vitro proliferating chicken PGCs can facilitate the production of efficient germline chimeras and transgenic chickens. Here, we describe methods to isolate and characterize PGCs from chicken embryos and their application to transgenesis.

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Acknowledgments

This work was supported by a National Research Foundation of Korea (NRF) grant, funded by the Korean government (MSIP; No. 2015R1A3A2033826), and the “Cooperative Research Program for Agriculture Science & Technology Development (Project No. PJ0122362016)” of the Rural Development Administration, Korea.

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Authors and Affiliations

  1. Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, South Korea

    Jae Yong Han & Bo Ram Lee

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  1. Jae Yong Han
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  2. Bo Ram Lee
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Corresponding author

Correspondence to Jae Yong Han .

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Editors and Affiliations

  1. IRCMS, Kumamoto University, Kumamoto, Kumamoto, Japan

    Guojun Sheng

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Han, J.Y., Lee, B.R. (2017). Isolation and Characterization of Chicken Primordial Germ Cells and Their Application in Transgenesis. In: Sheng, G. (eds) Avian and Reptilian Developmental Biology. Methods in Molecular Biology, vol 1650. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7216-6_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7216-6_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7215-9

  • Online ISBN: 978-1-4939-7216-6

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