Abstract
Small noncoding RNAs are essential for gene expression at transcriptional and posttranscriptional levels. Northern blot is the most used method for small RNA detection in tissues. Here we present an improved protocol for the Northern blot-based small RNA detection from plant tissues by using biotin-labeled probes. MicroRNAs and small interfering RNAs derived from Arabidopsis and Oryza sativa, respectively, have been detected with this methodology. Results suggest that this method is sensitive and efficient enough to detect small RNAs from plant tissues by using as low as 5 μg of total RNA. Furthermore, biotin-labeled probes are safer and easier to store for long term than radiolabeled probes.
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Acknowledgements
This work was supported by funds from National Natural Science Foundation of China (31371698 and 31670310).
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Hu, J., Zhu, Y. (2017). Nonradioactive Plant Small RNA Detection Using Biotin-Labeled Probes. In: Carbonell, A. (eds) Plant Argonaute Proteins. Methods in Molecular Biology, vol 1640. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7165-7_15
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DOI: https://doi.org/10.1007/978-1-4939-7165-7_15
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