Abstract
Gene-modified monkey models would be particularly valuable in biomedical and neuroscience research. Virus-based transgenic and programmable nucleases-based site-specific gene editing methods (TALEN, CRISPR-cas9) enable the generation of gene-modified monkeys with gain or loss of function of specific genes. Here, we describe the generation of transgenic and knock-out (KO) monkeys with high efficiency by lentivirus and programmable nucleases.
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References
Chan AW, Chong KY, Martinovich C, Simerly C, Schatten G (2001) Transgenic monkeys produced by retroviral gene transfer into mature oocytes. Science 291:309–312
Yang SH et al (2008) Towards a transgenic model of Huntington’s disease in a non-human primate. Nature 453:921–924
Sasaki E et al (2009) Generation of transgenic non-human primates with germline transmission. Nature 459:523–527
Liu Z et al (2016) Autism-like behaviours and germline transmission in transgenic monkeys overexpressing MeCP2. Nature 530:98–102
Liu Z et al (2014) Generation of a monkey with MECP2 mutations by TALEN-based gene targeting. Neurosci Bull 30:381–386
Liu H et al (2014) TALEN-mediated gene mutagenesis in rhesus and cynomolgus monkeys. Cell Stem Cell 14:323–328
Niu Y et al (2014) Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos. Cell 156:836–843
Sun Q et al (2008) Efficient reproduction of cynomolgus monkey using pronuclear embryo transfer technique. Proc Natl Acad Sci U S A 105:12956–12960
Bavister BD, Boatman DE, Collins K, Dierschke DJ, Eisele SG (1984) Birth of rhesus monkey infant after in vitro fertilization and nonsurgical embryo transfer. Proc Natl Acad Sci U S A 81:2218–2222
McKiernan SH, Bavister BD (2000) Culture of one-cell hamster embryos with water soluble vitamins: pantothenate stimulates blastocyst production. Hum Reprod 15:157–164
Wang H et al (2013) One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 153:910–918
Acknowledgment
This work was supported by grant “National Key Technology R&D Program of China 2014BAI03B00629(Q.S.)” and “Shanghai City Committee of science and technology project 14140900100 630 (Q.S.).”
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Liu, Z., Cai, Y., Sun, Q. (2017). Genome Editing of Monkey. In: Hatada, I. (eds) Genome Editing in Animals. Methods in Molecular Biology, vol 1630. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7128-2_12
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DOI: https://doi.org/10.1007/978-1-4939-7128-2_12
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7127-5
Online ISBN: 978-1-4939-7128-2
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