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Identification of Fungal T Cell Epitopes by Mass Spectrometry-Based Proteomics

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Book cover Vaccines for Invasive Fungal Infections

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1625))

Abstract

CD4+ T cells play a key role in host defense against many fungal infections. T cells are also implicated in vaccine immunity to fungi. To date, only a small number of fungal antigens have been identified. Knowing the antigenic determinants of fungi-specific T cells greatly facilitates the detection, enumeration and characterizes the antifungal T cells and it constitutes an important step toward the design and development of vaccination strategies. This chapter describes a method of MHC-II ligand elution and mass spectrometric analysis to identify naturally processed and presented fungal peptide epitopes.

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References

  1. Nanjappa SG, Heninger E, Wuthrich M, Gasper DJ, Klein BS (2012) Tc17 cells mediate vaccine immunity against lethal fungal pneumonia in immune deficient hosts lacking CD4+ T cells. PLoS Pathog 8:e1002771. doi:10.1371/journal.ppat.1002771

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Cassone A, Casadevall A (2012) Recent progress in vaccines against fungal diseases. Curr Opin Microbiol 15:427–433. doi:10.1016/j.mib.2012.04.004

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Schmidt CS, White CJ, Ibrahim AS, Filler SG, Fu Y, Yeaman MR et al (2012) NDV-3, a recombinant alum-adjuvanted vaccine for Candida and Staphylococcus aureus, is safe and immunogenic in healthy adults. Vaccine 30:7594–7600. doi:10.1016/j.vaccine.2012.10.038

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Lemmel C, Stevanovic S (2003) The use of HPLC-MS in T-cell epitope identification. Methods 29:248–259

    Article  CAS  PubMed  Google Scholar 

  5. Hillen N, Stevanovic S (2006) Contribution of mass spectrometry-based proteomics to immunology. Expert Rev Proteomics 3:653–664. doi:10.1586/14789450.3.6.653

    Article  CAS  PubMed  Google Scholar 

  6. Bär E, Gladiator A, Bastidas S, Roschitzki B, Acha-Orbea H, Oxenius A et al (2012) A novel Th cell epitope of Candida albicans mediates protection from fungal infection. J Immunol 188:5636–5643. doi:10.4049/jimmunol.1200594

    Article  PubMed  Google Scholar 

  7. Nesvizhskii AI, Keller A, Kolker E, Aebersold R (2003) A statistical model for identifying proteins by tandem mass spectrometry. Anal Chem 75:4646–4658

    Article  CAS  PubMed  Google Scholar 

  8. Wuthrich M, Brandhorst TT, Sullivan TD, Filutowicz H, Sterkel A, Stewart D et al (2015) Calnexin induces expansion of antigen-specific CD4(+) T cells that confer immunity to fungal ascomycetes via conserved epitopes. Cell Host Microbe 17:452–465. doi:10.1016/j.chom.2015.02.009

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Moon JJ, Chu HH, Pepper M, McSorley SJ, Jameson SC, Kedl RM et al (2007) Naive CD4(+) T cell frequency varies for different epitopes and predicts repertoire diversity and response magnitude. Immunity 27:203–213. doi:10.1016/j.immuni.2007.07.007

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Perruccio K, Tosti A, Burchielli E, Topini F, Ruggeri L, Carotti A et al (2005) Transferring functional immune responses to pathogens after haploidentical hematopoietic transplantation. Blood 106:4397–4406. doi:10.1182/blood-2005-05-1775

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Haque T, Wilkie GM, Taylor C, Amlot PL, Murad P, Iley A et al (2002) Treatment of Epstein-Barr-virus-positive post-transplantation lymphoproliferative disease with partly HLA-matched allogeneic cytotoxic T cells. Lancet 360:436–442. doi:10.1016/S0140-6736(02)09672-1

    Article  PubMed  Google Scholar 

  12. Papadopoulou A, Gerdemann U, Katari UL, Tzannou I, Liu H, Martinez C et al (2014) Activity of broad-spectrum T cells as treatment for AdV, EBV, CMV, BKV, and HHV6 infections after HSCT. Sci Transl Med 6:242ra83. doi:10.1126/scitranslmed.3008825

    Article  PubMed  PubMed Central  Google Scholar 

  13. Peggs KS, Thomson K, Samuel E, Dyer G, Armoogum J, Chakraverty R et al (2011) Directly selected cytomegalovirus-reactive donor T cells confer rapid and safe systemic reconstitution of virus-specific immunity following stem cell transplantation. Clin Infect Dis 52:49–57. doi:10.1093/cid/ciq042

    Article  CAS  PubMed  Google Scholar 

  14. Steiner QG, Otten LA, Hicks MJ, Kaya G, Grosjean F, Saeuberli E et al (2008) In vivo transformation of mouse conventional CD8alpha+ dendritic cells leads to progressive multisystem histiocytosis. Blood 111:2073–2082. doi:10.1182/blood-2007-06-097576

    Article  CAS  PubMed  Google Scholar 

  15. Mommen GPM, Frese CK, Meiring HD, van Gaans-van den Brink J, de Jong APJM, van Els CACM et al (2014) Expanding the detectable HLA peptide repertoire using electron-transfer/higher-energy collision dissociation (EThcD). Proc Natl Acad Sci U S A 111:4507–4512. doi:10.1073/pnas.1321458111

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

We would like to thank Prof. Stefan Stevanović for technical advice and suggestions. Work in the LeibundGut-laboratory is supported by the Swiss National Science Foundation, the Gebert Rüf Foundation, the Promedica Foundation, and the University of Zürich.

Author information

Authors and Affiliations

  1. Functional Genomics Center Zürich, University of Zürich & ETH Zurich, Winterthurerstrasse 190, CH-8057, Zürich, Switzerland

    Bernd Roschitzki

  2. Section of Immunology, Vetsuisse Faculty, University of Zürich, Winterthurerstrasse 266a, CH-8057, Zürich, Switzerland

    Salomé LeibundGut-Landmann

Authors
  1. Bernd Roschitzki
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  2. Salomé LeibundGut-Landmann
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Corresponding author

Correspondence to Salomé LeibundGut-Landmann .

Editor information

Editors and Affiliations

  1. Department of Molecular Immunology, Beckman Research Institute of City of Hope, Duarte, California, USA

    Markus Kalkum

  2. Department of Molecular Immunology, Beckman Research Institute of City of Hope, Duarte, California, USA

    Margarita Semis

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Roschitzki, B., LeibundGut-Landmann, S. (2017). Identification of Fungal T Cell Epitopes by Mass Spectrometry-Based Proteomics. In: Kalkum, M., Semis, M. (eds) Vaccines for Invasive Fungal Infections. Methods in Molecular Biology, vol 1625. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7104-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7104-6_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7103-9

  • Online ISBN: 978-1-4939-7104-6

  • eBook Packages: Springer Protocols

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