Abstract
Food safety and quality are nowadays a major consumers’ concern. In the dairy industry the fraudulent addition of cheaper/lower-quality milks from nonlegitimate species/breeds compromises the quality and value of the final product. Despite the already existing approaches for identification of the species origin of milk, there is little information regarding differentiation at an intra-species level. In this protocol we describe a low-cost, sensitive, fast, and reliable analytical technique—Random Amplified Polymorphic DNA/Sequence Characterized Amplified Region (RAPD/SCAR)—capable of an efficient detection of adulterant breeds in milk mixtures used for fraudulent manufacturing of dairy products and suitable for the detection of milk adulteration in processed dairy foods.
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Acknowledgments
This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684), BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020—Programa Operacional Regional do Norte, and project “Valor Queijo” (CENTRO-07-0202-FEDER-030372) funded by FCT/MCTES (PIDDAC) and cofunded by “Fundo Europeu de Desenvolvimento Regional” (FEDER) through “COMPETE—Programa Operacional Factores de Competitividade” (POFC).
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Cunha, J.T., Domingues, L. (2017). RAPD/SCAR Approaches for Identification of Adulterant Breeds’ Milk in Dairy Products. In: Domingues, L. (eds) PCR. Methods in Molecular Biology, vol 1620. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-7060-5_13
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DOI: https://doi.org/10.1007/978-1-4939-7060-5_13
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