Abstract
Mesenchymal stem cells (MSCs), together with hematopoietic stem cells (HSCs), are the most frequently used cell type for cell-based therapeutics. As for other cell types intended for research and translational use, it is important to establish correctly typed cell lines from human tissue donations. Here, we describe methods for isolating, culturing, and identifying MSCs from various tissues obtained through human tissue donation. The methods have been used in the context of a biobank, prepared as standard operating procedures (SOPs), ensuring traceability and reproducibility of cell production.
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Ferrin, I., Beloqui, I., Zabaleta, L., Salcedo, J.M., Trigueros, C., Martin, A.G. (2017). Isolation, Culture, and Expansion of Mesenchymal Stem Cells. In: Crook, J., Ludwig, T. (eds) Stem Cell Banking. Methods in Molecular Biology, vol 1590. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6921-0_13
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DOI: https://doi.org/10.1007/978-1-4939-6921-0_13
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Publisher Name: Humana Press, New York, NY
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