Abstract
Suspension-cultured cells respond sensitively to a number of stimuli including pathogen-derived molecules. Therefore, they are used in a simple, single-cell model system in order to gain insights into immune signaling pathways in rice. Here we describe protocols for studying chitin-induced MAPK activation and ROS generation in rice suspension-cultured cells.
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Acknowledgments
This research was supported by Grants-in-Aid for Scientific Research (A)(15H02489), for Scientific Research on Innovative Areas (25114517 and 15H01242), and by the Strategic Project to Support the Formation of Research Bases at Private Universities: Matching Fund Subsidy from the Ministry of Education, Culture, Sports, Science and Technology, 2011-2015 (S1101035) to T.K., and Grants-in-Aid for Scientific Research (15K18649) and Basic Science Research Projects from Sumitomo Foundation to K.Y.
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Yamaguchi, K., Kawasaki, T. (2017). Chitin-Triggered MAPK Activation and ROS Generation in Rice Suspension-Cultured Cells. In: Shan, L., He, P. (eds) Plant Pattern Recognition Receptors. Methods in Molecular Biology, vol 1578. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6859-6_26
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DOI: https://doi.org/10.1007/978-1-4939-6859-6_26
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