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Spectroscopic Photoacoustic Imaging of Gold Nanorods

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Biomedical Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1570))

Abstract

Photoacoustic imaging is a rapidly developing tool capable of achieving high-resolution images with optical contrast at imaging depths up to a few centimeters. When combined with targeted nanoparticle contrast agents, sensitive detection of molecular signatures is possible. In this chapter, we discuss the achievements and future directions of nanoparticle-augmented photoacoustic imaging. We present a method to synthesize silica-coated gold nanorods, which are highly stable, signal amplifying photoacoustic contrast agents, and also describe spectroscopic image acquisition and processing steps to provide a specific map of nanoparticle distribution in vivo.

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Acknowledgments

The methods described in this chapter build on the excellent work of and discussions with Dr. Stanislav Emelianov of Georgia Institute of Technology, Dr. Kimberly Homan of Nanohybrids Inc., Dr. Yun-Sheng Chen of Stanford University, and Dr. Seungsoo Kim of Siemens Healthcare.

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Authors and Affiliations

  1. Thayer School of Engineering, Dartmouth College, 14 Engineering Drive, Hanover, NH, 03755, USA

    Austin Van Namen & Geoffrey P. Luke

Authors
  1. Austin Van Namen
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  2. Geoffrey P. Luke
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Corresponding author

Correspondence to Geoffrey P. Luke .

Editor information

Editors and Affiliations

  1. Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, Illinois, USA

    Sarah Hurst Petrosko

  2. Department of Biomedical Engineering, University of Delaware, Newark, Delaware, USA

    Emily S. Day

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Namen, A.V., Luke, G.P. (2017). Spectroscopic Photoacoustic Imaging of Gold Nanorods. In: Petrosko, S., Day, E. (eds) Biomedical Nanotechnology. Methods in Molecular Biology, vol 1570. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6840-4_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6840-4_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6838-1

  • Online ISBN: 978-1-4939-6840-4

  • eBook Packages: Springer Protocols

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