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Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration

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Histochemistry of Single Molecules

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1560))

Abstract

Muscle stem cells, named satellite cells, are quiescent in resting skeletal muscle. Following injury, satellite cells are activated and become proliferating myoblasts that either self-renew or differentiate. Several markers are used to identify the different myogenic populations, such as Pax7 (quiescent and activated satellite cells), MyoD (proliferating myoblasts), and myogenin (differentiating myoblasts). Immunodetection of these markers is a very useful tool to analyze myogenic cells and muscle regeneration. Here, we describe a method for immunodetection of satellite cells and their myogenic progeny in resting and regenerating skeletal muscles.

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Author information

Authors and Affiliations

  1. Centre for Stem Cell Research, Ottawa Hospital Research Institute, 501 Smyth Road, Ottawa, ON, Canada, K1H 8L6

    Nicolas A. Dumont & Michael A. Rudnicki

  2. Faculty of Medicine, Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON, Canada

    Nicolas A. Dumont & Michael A. Rudnicki

  3. Sainte-Justine Hospital Research Center, and Department of Rehabilitation, Faculty of Medicine, University of Montreal, Montreal, QC, Canada

    Nicolas A. Dumont

Authors
  1. Nicolas A. Dumont
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  2. Michael A. Rudnicki
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Corresponding author

Correspondence to Michael A. Rudnicki .

Editor information

Editors and Affiliations

  1. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Carlo Pellicciari

  2. Department of Biology and Biotechnology, University of Pavia, Pavia, Italy

    Marco Biggiogera

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Dumont, N.A., Rudnicki, M.A. (2017). Characterizing Satellite Cells and Myogenic Progenitors During Skeletal Muscle Regeneration. In: Pellicciari, C., Biggiogera, M. (eds) Histochemistry of Single Molecules. Methods in Molecular Biology, vol 1560. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6788-9_12

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  • DOI: https://doi.org/10.1007/978-1-4939-6788-9_12

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6787-2

  • Online ISBN: 978-1-4939-6788-9

  • eBook Packages: Springer Protocols

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