Abstract
Mesenchymal stromal cells (MSCs) and induced pluripotent stem cells (iPSCs) have stimulated much interest in the scientific community and hopes among the general public since their discovery in 1966 due to a variety of potential applications it has in the field of regenerative medicine. Copious amount of literature, as well as long-term animal and human clinical trials, indicates that MSCs can be successfully used for therapeutic purpose without any extreme adversities. MSCs have been isolated from adult and fetal tissues. Recently, MSCs from placenta have generated much inquisitiveness. In this article, we will demonstrate the step-by-step procedure for isolating human placental MSCs from term placenta, reprogramming of placental MSCs into iPSCs using plasmid vectors, evaluation of functional recovery in mice spinal cord injury models, and in vivo tracking of the transplanted cells.
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References
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Acknowledgments
We are grateful to Department of Biotechnology (DBT), Government of India for Ramalingaswami Fellowship and research support grants (DBT Grants BT/PR8527/MED/31/234/2013; BT/PR8742/AGR/36/773/2013; and BT/PR 15420/MED/31/122/2011) to Sanjay Kumar. We acknowledge and thank Indian Council of Medical Research (ICMR) for Junior/Senior Research Fellowship(s) to Franklin Jebaraj Herbert. We would like to thank Dr. Anne George Cherian, Department of Obstetrics & Gynaecology and Dr. George Tharion, Department of Physical Medicine & Rehabilitation for valuable assistance during research.
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Sabapathy, V., Herbert, F.J., Kumar, S. (2017). Therapeutic Application of Placental Mesenchymal Stem Cells Reprogrammed Neurospheres in Spinal Cord Injury of SCID. In: Di Nardo, P., Dhingra, S., Singla, D. (eds) Adult Stem Cells. Methods in Molecular Biology, vol 1553. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6756-8_8
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DOI: https://doi.org/10.1007/978-1-4939-6756-8_8
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6756-8
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