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Using Electrophoretic Immunoassay to Monitor Hormone Secretion

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Microchip Diagnostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1547))

Abstract

It has been demonstrated that microfluidic systems allow integration of sampling, reagent mixing, and rapid electrophoretic analysis. They have also proven useful for culturing cells wherein control over the environment allows novel and automated experiments. Here, we describe a microchip-based electrophoresis assay that allows cell culture and hormone monitoring. An online gradient generator can control cell culture condition precisely. This system has been applied for Pancreas islets’ glucose sensitivity studies.

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Author information

Authors and Affiliations

  1. Department of Chemistry, University of Michigan, 930 N. University Avenue, Ann Arbor, MI, 48109, USA

    Shusheng Lu & Robert T Kennedy

  2. Department of Pharmacology, University of Michigan, 1150 W. Medical Center Drive, Ann Arbor, MI, 48109, USA

    Robert T Kennedy

Authors
  1. Shusheng Lu
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  2. Robert T Kennedy
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Corresponding author

Correspondence to Robert T Kennedy .

Editor information

Editors and Affiliations

  1. INSERM UMRS1147, CNRS SNC 5014, Université Paris Descartes, Equipe labellisée Ligue Nationale contre le cancer 2016, Paris, France

    Valérie Taly

  2. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

    Jean-Louis Viovy

  3. Macromolecules and Microsystems in Biology and Medicine, Institut Curie, Centre National de la Recherche Scientifique, Université Pierre et Marie Curie, PSL Research University, UMR 168, Paris, France

    Stéphanie Descroix

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Lu, S., Kennedy, R.T. (2017). Using Electrophoretic Immunoassay to Monitor Hormone Secretion. In: Taly, V., Viovy, JL., Descroix, S. (eds) Microchip Diagnostics. Methods in Molecular Biology, vol 1547. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6734-6_5

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  • DOI: https://doi.org/10.1007/978-1-4939-6734-6_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6732-2

  • Online ISBN: 978-1-4939-6734-6

  • eBook Packages: Springer Protocols

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