Abstract
Digital nucleic acid amplification (Digital NAA) quantifies nucleic acid by compartmentalizing a sample of DNA or RNA into a large number of discrete partitions and performing parallel nucleic acid amplification, such as polymerase chain reaction (PCR) or isothermal amplification reactions. With the counts of positive wells, total number of wells, and volumes of wells, the concentration of the target nucleic acid in the sample can be quantified. Digital NAA is considered increasingly powerful for ultra-sensitive detection and accurate quantification of nucleic acid for biological research and potentially medical diagnostics. Here, we describe glass SlipChip devices to perform digital NAA without cumbersome manual manipulation or complex fluidic control systems.
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Shen, F. (2017). SlipChip Device for Digital Nucleic Acid Amplification. In: Taly, V., Viovy, JL., Descroix, S. (eds) Microchip Diagnostics. Methods in Molecular Biology, vol 1547. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6734-6_10
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DOI: https://doi.org/10.1007/978-1-4939-6734-6_10
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6734-6
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