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Imaging Techniques in Insects

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Lateralized Brain Functions

Part of the book series: Neuromethods ((NM,volume 122))

Abstract

The present chapter describes how to apply optical neuroimaging to study brain lateralization in insects. It provides two complete protocols, one for in vivo imaging to obtain information on functional lateralization, and one on histochemical techniques to study morphological asymmetries. Both sections start with the animal preparation, and illustrate the different possibilities for brain tissue labeling. Then, imaging techniques are presented, concentrating on wide-field fluorescence microscopy, confocal, and two-photon laser scanning microscopy. After some remarks on the main methods for data analysis, studies on functional and morphological lateralization in insects are reviewed.

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Authors and Affiliations

  1. Center for Mind/Brain Sciences, University of Trento, Rovereto, Italy

    Marco Paoli, Mara Andrione & Albrecht Haase

  2. Department of Physics, University of Trento, Trento, Italy

    Albrecht Haase

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  1. Marco Paoli
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  2. Mara Andrione
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  3. Albrecht Haase
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Correspondence to Marco Paoli .

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Editors and Affiliations

  1. School of Science and Technology, University of New England, Armidale, Australia

    Lesley J. Rogers

  2. Centre for Mind/Brain Sciences, University of Trento, Trento, Trento, Italy

    Giorgio Vallortigara

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Paoli, M., Andrione, M., Haase, A. (2017). Imaging Techniques in Insects. In: Rogers, L., Vallortigara, G. (eds) Lateralized Brain Functions. Neuromethods, vol 122. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6725-4_15

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  • DOI: https://doi.org/10.1007/978-1-4939-6725-4_15

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