Abstract
A multiplex competitive antibody-based assay in a dipstick format for the simultaneous detection of major of Fusarium mycotoxins in oats is described. Ground oats sample is extracted with a mixture of methanol and water. Sample extract is diluted and directly analyzed by a multiplex dipstick. The test kit employs a microwell of reagents containing antibodies linked to gold particles and a dipstick made up of a nitrocellulose membrane were specific capture lines are located. In the presence of oats extract each antibody binds the corresponding mycotoxin before starting to run vertically on the dipstick in the direction of the capture lines. In 10 min red lines rise from the background on the dipstick. Results are interpreted by an optical reader measuring the ratio between each test line and a control line located on the top of the strip.
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Acknowledgements
The research leading to these results has received funding from the European Commission’s Seventh Framework Programme (FP7/2007–2013) under grant agreement no. 211326.
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Lattanzio, V.M.T., Nivarlet, N. (2017). Multiplex Dipstick Immunoassay for Semiquantitative Determination of Fusarium Mycotoxins in Oat. In: Gasparis, S. (eds) Oat. Methods in Molecular Biology, vol 1536. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6682-0_10
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DOI: https://doi.org/10.1007/978-1-4939-6682-0_10
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