Skip to main content

Mining Plant Genomic and Genetic Data Using the GnpIS Information System

  • Protocol
  • First Online:
Plant Genomics Databases

Abstract

GnpIS is an information system designed to help scientists working on plants and fungi to decipher the molecular and genetic architecture of trait variations by facilitating the navigation through genetic, genomic, and phenotypic information. The purpose of the present chapter is to illustrate how users can (1) explore datasets from phenotyping experiments in order to build new datasets for studying genotype × environment interactions in traits, (2) browse into the results of other genetic analysis data such as GWAS to generate or check working hypothesis about candidate genes or to identify important alleles and germplasms for breeding programs, and (3) explore the polymorphism in specific area of the genome using InterMine, JBrowse tools embedded in the GnpIS information system.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. http://ec.europa.eu/research/participants/data/ref/h2020/grants_manual/hi/oa_pilot/h2020-hi-oa-pilot-guide_en.pdf

  2. Kaiser J (2015) U.S. agencies fall in line on public access. Science 349:167

    Article  Google Scholar 

  3. Steinbach D, Alaux M, Amselem J, Choisne N, Durand S, Flores R, Keliet AO, Kimmel E, Lapalu N, Luyten I, Michotey C, Mohellibi N, Pommier C, Reboux S, Valdenaire D, Verdelet D, Quesneville H (2013) GnpIS: an information system to integrate genetic and genomic data from plants and fungi. Database (Oxford) 2013:Bat058. doi:10.1093/database/bat058

    Article  Google Scholar 

  4. Krajewski P, Chen D, Ćwiek H, van Dijk ADJ, Fiorani F, Kersey P, Klukas C, Lange M, Markiewicz A, Nap JP, van Oeveren J, Pommier C, Scholz U, van Schriek M, Usadel B, Weise S (2015) Towards recommendations for metadata and data handling in plant phenotypic experiments. J Exp Bot 66(18):5417–5427. doi:10.1093/jxb/erv271

    Article  CAS  PubMed  Google Scholar 

  5. Skinner ME, Uzilov AV, Stein LD, Mungall CJ, Holmes IH (2009) JBrowse: a next-generation genome browser. Genome Res 19(9):1630–1638. doi:10.1101/gr.094607.109

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. http://www.bioversityinternational.org/e-library/publications/detail/faobioversity-multi-crop-passport-descriptors-v2-mcpd-v2/

  7. Smith RN, Aleksic J, Butano D, Carr A, Contrino S, Hu F, Lyne M, Lyne R, Kalderimis A, Rutherford K, Stepan R, Sullivan J, Wakeling M, Watkins X, Micklem G (2012) InterMine: a flexible data warehouse system for the integration and analysis of heterogeneous biological data. Bioinformatics 28(23):3163–3165. doi:10.1093/bioinformatics/bts577

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Oury FX, Godin C, Mailliard A, Chassin A, Gardet O, Giraud A, Heumez E, Morlais JY, Rolland B, Rousset M, Trottet M, Charmet G (2012) A study of genetic progress due to selection reveals a negative effect of climate change on bread wheat yield in France. Eur J Agronomy 40:28–38. doi:10.1016/j.eja.2012.02.007

    Article  Google Scholar 

  9. Sauvage C, Segura V, Bauchet G, Stevens R, Do PT, Nikoloski Z, Fernie AR, Causse M (2014) Genome-wide association in tomato reveals 44 candidate loci for fruit metabolic traits. Plant Physiol 165:1120–1132. doi:10.1104/pp.114.241521

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Houel C, Bounon R, Chaïb J, Guichard C, Péros JP, Bacilieri R, Dereeper A, Canaguier A, Lacombe T, N’Diaye A, Le Paslier MC, Vernerey MS, Coriton O, Brunel D, This P, Torregrosa L, Adam-Blondon AF (2010) Patterns of sequence polymorphism in the fleshless berry locus in cultivated and wild Vitis vinifera accessions. BMC Plant Biol 10:284. doi:10.1186/1471-2229-10-284

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Adam-Blondon A-F, Jaillon O, Vezzulli S, Zharkikh A, Troggio M, Velasco R (2011) Genome Sequence Initiatives. In: Adam-Blondon A-F, Martinez-Zapater JM, Kole C (eds) Genetics, genomics and breeding of grapes. Science Publishers, Enfield, pp 211–234. ISBN 9781578087174

    Chapter  Google Scholar 

Download references

Acknowledgments

We gratefully thank Aminah-Olivia Keliet, Btissam Aissaoui, Laura Burlot, Loic Couderc, Guillaume Cornut, Mathieu Labernardière, Mathilde Lainé, Aristide Lebreton, Florian Philippe, Sandrine Nsigue-Meilo, and Daphnée Verdelet for their help in some developments, data insertion, and trainings on GnpIS in the last 4 years. We also warmly thank our past and present projects’ partners who are providing the data inserted in the database and who are at the origin of many improvements. For the most important recent improvements, we specially thank Stephane Nicolas, Mathilde Causse, Christopher Sauvage, Jacques Le Gouis, Alain Charcosset, Patrice This, Thierry Lacombe, Gilles Charmet, François-Xavier Oury, Arnaud Gauffreteau, Etienne Paux, and Frédéric Choulet. GnpIS has been developed in the last 4 years with the support of INRA, the ANR p rojects of the “Investment for the Future” call Phenome (https://www.phenome-fppn.fr/), Aker (http://www.aker-betterave.fr/en/), Amaizing (ANR-10-BTBR-03), Breedwheat (ANR-10-BTBR-02) and Peamust (ANR-11-BTBR-02), the ANR project GnpAsso (ANR-10-GENM-0006), the TransPLANT FP7 European infrastructure project (project no 283496).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to A.-F. Adam-Blondon .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2017 Springer Science+Business Media New York

About this protocol

Cite this protocol

Adam-Blondon, AF. et al. (2017). Mining Plant Genomic and Genetic Data Using the GnpIS Information System. In: van Dijk, A. (eds) Plant Genomics Databases. Methods in Molecular Biology, vol 1533. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6658-5_5

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-6658-5_5

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6656-1

  • Online ISBN: 978-1-4939-6658-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics