Abstract
Since the early 1950s, vascular reactivity using isolated vessel rings has been a useful and efficient model for physiological and pharmacological studies. This experimental model was utilized in the milestone study of Dr. Robert Furchgott to discover the endothelium-derived relaxation factor (EDRF) nitric oxide (NO), opening new avenues and scientific perspectives in the vascular pathophysiology. Moreover, the isolated vessel ring preparation had an important contribution to understand many vasoactive systems. Here, we described the isolated aorta technique and pitfalls about the use of angiotensin-(1-7) peptide in this preparation.
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Acknowledgment
This work was supported by the National Institute of Science and Technology in Nanobiopharmaceutics, Brazil.
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Lautner, R.Q., Fraga-Silva, R.A., Ferreira, A.J., Santos, R.A.S. (2017). Vascular Reactivity of Isolated Aorta to Study the Angiotensin-(1-7) Actions. In: Touyz, R., Schiffrin, E. (eds) Hypertension. Methods in Molecular Biology, vol 1527. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6625-7_29
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DOI: https://doi.org/10.1007/978-1-4939-6625-7_29
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