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Cdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic Regions

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The Mitotic Exit Network

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1505))

Abstract

Chromosome condensation is an essential morphological event required for successful DNA segregation during mitosis. The high level of genome compaction achieved during this process is attained by the evolutionary conserved condensin complex. Recently, several lines of evidences have demonstrated that the mitotic phosphatase Cdc14 is required to ensure condensin loading onto chromosomes. To date several approaches have been used in order to characterize condensin activity and regulation, however these techniques are time-consuming and require complex equipment. In this chapter we described an easy and reliable protocol to analyze Cdc14-dependent condensin loading onto specific genomic DNA regions by using a chromatin immunoprecipitation (ChIP) technique.

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Author information

Authors and Affiliations

  1. Cell Cycle and Genome Stability Group, Instituto de Biología Funcional y Genómica, Consejo Superior de Investigaciones Científicas (CSIC), Universidad de Salamanca (USAL), C/ Zacarías González 2, Salamanca, 37007, Spain

    Facundo Ramos & Andrés Clemente-Blanco

  2. Cell Cycle Group, Medical Research Council, Clinical Sciences Centre, Imperial College London, Du Cane Road, London, W12 0NN, UK

    Joanne Leonard & Luis Aragón

Authors
  1. Facundo Ramos
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  2. Joanne Leonard
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  3. Andrés Clemente-Blanco
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  4. Luis Aragón
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Corresponding author

Correspondence to Luis Aragón .

Editor information

Editors and Affiliations

  1. CABIMER, Sevilla, Spain

    Fernando Monje-Casas

  2. Cancer Epigenetics & Biology Program, IDIBELL Hospitalet de Llobregat, Barcelona, Spain

    Ethel Queralt

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Ramos, F., Leonard, J., Clemente-Blanco, A., Aragón, L. (2017). Cdc14 and Chromosome Condensation: Evaluation of the Recruitment of Condensin to Genomic Regions. In: Monje-Casas, F., Queralt, E. (eds) The Mitotic Exit Network. Methods in Molecular Biology, vol 1505. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6502-1_17

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  • DOI: https://doi.org/10.1007/978-1-4939-6502-1_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-6500-7

  • Online ISBN: 978-1-4939-6502-1

  • eBook Packages: Springer Protocols

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