Abstract
Gametocidal (Gc) system is a novel method of inducing chromosome mutations in wheat by using Gc genes of wheat-related species. Many deletion lines were efficiently produced by this system and used for physical mapping of genes and DNA markers. A large stocks with chromosome deletions in homozygous state as well as the lines with Gc genes are available in the gene bank of Japan (KOMUGI). In this chapter, I describe the method of inducing breakage in a target chromosome using lines with the Gc gene on chromosome 2C of Aegilops cylindrica (a wheat-related wild species) and nullisomic–tetrasomics.
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References
Tsujimoto H (2005) Gametocidal genes in wheat as the inducer of chromosome breakage. Wheat Inf Serv 100:33–48
Tsujimoto H, Yamada T, Hasegawa K, Usami T, Endo TR, Ogihara Y, Sasakuma T (2001) Large-scale selection of lines with deletion in chromosome 1B in wheat and application for fine deletion mapping. Genome 44:501–508
Endo TR, Gill BS (1996) The deletion stocks of common wheat. J Hered 87:295–307
International Wheat Genome Sequence Consortium (2014) A chromosome-based draft sequence of the hexaploid bread wheat (Triticum aestivum) genome. Science 345:1251788
Endo TR (2009) Cytological dissection of barley genome by gametocidal system. Breed Sci 59:481–486
Li JJ, Nasuda S, Endo TR (2013) Dissection of rye chromosomes by gametocidal system. Genes Genet Syst 88:321–327
Nasuda S, Kikkawa Y, Ashida T, Rafiqul Islam AKM, Sato K, Endo TR (2005) Chromosomal assignment and deletion mapping of barley EST markers. Genes Genet Syst 80:357–366
Acknowledgment
I thank Prof. Benjamin Ubi, Ebonyi State University, Nigeria, for reading and editing the manuscript.
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© 2016 Springer Science+Business Media New York
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Tsujimoto, H. (2016). Gametocidal System for Dissecting Wheat Chromosomes. In: Murata, M. (eds) Chromosome and Genomic Engineering in Plants. Methods in Molecular Biology, vol 1469. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-4931-1_7
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DOI: https://doi.org/10.1007/978-1-4939-4931-1_7
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-4931-1
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