Abstract
Genetically homogeneous populations such as inbred strains are valuable experimental tools in various fields of biomedical analyses. In many animals, inbred strains are established by consecutive sib-pair mating for a minimum of 20 generations. As the generation proceeds, fitness of the population reduces usually. Therefore, in order to establish inbred strains, the important point is the selection of pairs in good condition at each generation. Here, I describe the procedure and tips for generating inbred strains in zebrafish.
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Acknowledgment
I thank Dr. Noriyoshi Sakai and his lab members in the National Institute of Genetics for their valuable assistance in breeding zebrafish. I am also grateful to Ms. Hideko Utsumi, Dr. Taijiro Yabe, and Prof. Shinji Takada for their kind support in the maintenance of the IM strain and Drs. Atsuko Shimada, Kiyoshi Naruse, Yasuhiro Kamei, Tomonori Deguchi, and Koichi Kawakami for their helpful support and discussions. The work was supported partly by Grant-in-Aid for Young Scientists (B) from Japan Society for the Promotion of Science, by the Center for the Promotion of Integrated Sciences (CPIS) of Sokendai, and by NIBB Collaborative Research Program (c-12-351, b-13-202).
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Shinya, M. (2016). Construction of the Inbred Strain. In: Kawakami, K., Patton, E., Orger, M. (eds) Zebrafish. Methods in Molecular Biology, vol 1451. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3771-4_8
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DOI: https://doi.org/10.1007/978-1-4939-3771-4_8
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-3771-4
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