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Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay

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Human Respiratory Syncytial Virus

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1442))

Abstract

One of the most commonly used approaches for determining the quantity of infectious RSV particles in a given sample is the plaque assay. RSV infectious particles can be quantified by various direct and indirect methods. Here, we explain two simple methods for RSV titration: plaque assay and immunostaining assay.

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References

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Correspondence to Ralph A. Tripp Ph.D. .

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Jorquera, P.A., Tripp, R.A. (2016). Quantification of RSV Infectious Particles by Plaque Assay and Immunostaining Assay. In: Tripp, R., Jorquera, P. (eds) Human Respiratory Syncytial Virus. Methods in Molecular Biology, vol 1442. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3687-8_3

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  • DOI: https://doi.org/10.1007/978-1-4939-3687-8_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3685-4

  • Online ISBN: 978-1-4939-3687-8

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