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Investigating Bacterial Chromosome Architecture

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Chromosome Architecture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1431))

Abstract

How is the bacterial chromosome organized within the bacterial cell? Over the last 60 years, a variety of approaches have been used to investigate this question. More recently, the parallel development of epifluorescence microscopy and genetic tools has enabled the direct visualization of the intracellular positioning of DNA sequences in live cells and has consequently revolutionized our view of the architecture of the nucleoid in vivo. In this chapter I present a comprehensive methodology designed to characterize the architecture of the nucleoid DNA and the positioning of specific DNA sequences in live Escherichia coli cells. DNA localization systems, preparation of stable agarose-mounted microscopy slides, and basic image analysis tools are mentioned.

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Acknowledgments

Thanks to Nelly Dubarry for critical reading. This work was supported by ATIP-Avenir 2014 grant (CNRS and INSERM) and Finovi Funding to Christian Lesterlin.

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Authors and Affiliations

  1. MMSB - Molecular Microbiology and Structural Biochemistry, Université Lyon 1, CNRS, UMR 5086, 7 Passage du Vercors, 69 367, Lyon Cedex 07, France

    Christian Lesterlin & Nelly Duabrry

Authors
  1. Christian Lesterlin
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  2. Nelly Duabrry
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Corresponding authors

Correspondence to Christian Lesterlin or Nelly Duabrry .

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Editors and Affiliations

  1. Biological Physical Sciences Instit, University of York, York, United Kingdom

    Mark C. Leake

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Lesterlin, C., Duabrry, N. (2016). Investigating Bacterial Chromosome Architecture. In: Leake, M. (eds) Chromosome Architecture. Methods in Molecular Biology, vol 1431. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3631-1_6

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  • DOI: https://doi.org/10.1007/978-1-4939-3631-1_6

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3629-8

  • Online ISBN: 978-1-4939-3631-1

  • eBook Packages: Springer Protocols

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