Abstract
In vitro-synthesized mRNA containing nucleoside modifications has great therapeutical potential to transiently express proteins with physiological importance. One such protein is photolyase which rapidly removes UV-induced DNA damages, but this enzyme is absent in humans. Here, we apply a novel mRNA-based platform to achieve functional nonhuman photolyase production in cultured human keratinocytes. Transfection of nucleoside-modified mRNA encoding photolyase leads to accelerated repair of DNA photolesions in human keratinocytes.
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Acknowledgements
This work was supported by National Institutes of Health (grant number R01NS029331 and R42HL87688), the Hungarian Scientific Research Fund (OTKA K105872), and the project of TÁMOP-4.2.2-A-11/1/KONV-2012-0031.
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Boros, G. et al. (2016). Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage. In: Rhoads, R. (eds) Synthetic mRNA. Methods in Molecular Biology, vol 1428. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3625-0_14
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DOI: https://doi.org/10.1007/978-1-4939-3625-0_14
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