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Cross-Presentation Assay for Human Dendritic Cells

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Dendritic Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1423))

Abstract

The presentation of exogenous antigens on major histocompatibility complex (MHC) class I molecules, termed cross-presentation, is essential for the initiation of cytotoxic immune responses. Numerous studies in mice and human have shown that dendritic cells are the best cross-presenting cells. The protocol described here allows the assessment of the cross-presentation by human dendritic cells of a model antigen (either soluble or cell associated) to antigen-specific CD8 T cells.

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Acknowledgments

This work is supported by INSERM, the European Research Council, Labex DCBIOL, and Ligue contre le Cancer.

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Authors and Affiliations

  1. INSERM U932, and Institut Curie, Centre de recherche, 26 rue d’Ulm, 75248, Paris Cedex 05, France

    Elodie Segura

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  1. Elodie Segura
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Correspondence to Elodie Segura .

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Editors and Affiliations

  1. Pavillon Pasteur, Institut Curie INSERM U932, Paris Cedex 05, France

    Elodie Segura

  2. Department of Biodefense, Tokyo Medical and Dental University, Tokyo, Japan

    Nobuyuki Onai

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Segura, E. (2016). Cross-Presentation Assay for Human Dendritic Cells. In: Segura, E., Onai, N. (eds) Dendritic Cell Protocols. Methods in Molecular Biology, vol 1423. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3606-9_14

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  • DOI: https://doi.org/10.1007/978-1-4939-3606-9_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3604-5

  • Online ISBN: 978-1-4939-3606-9

  • eBook Packages: Springer Protocols

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