Abstract
Murine models of intestinal inflammation have been widely used in biomedical research. Similarities in anatomy and physiology between such murine models and patients with inflammatory bowel diseases may allow a better understanding of the pathogenesis of Crohn’s disease and ulcerative colitis. Additionally, models of intestinal inflammation may be used for the analysis of potentially new therapeutic agents. One key class of models consists of chemically induced inflammation models. Within this group, colitis induced by the haptenizing agent oxazolone is an important model that results in induction of acute or chronic inflammation of the large bowel. Here, we describe the induction and the analysis of this experimental colitis model.
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References
Strober W, Fuss I, Mannon P (2007) The fundamental basis of inflammatory bowel disease. J Clin Invest 117:514–521
Strober W, Fuss IJ, Blumberg RS (2002) The immunology of mucosal models of inflammation. Annu Rev Immunol 20:495–549
Wirtz S, Neurath MF (2000) Animal models of intestinal inflammation: new insights into the molecular pathogenesis and immunotherapy of inflammatory bowel disease. Int J Colorectal Dis 15:144–160
Ina D, Pallone F (2008) What is the role of cytokines and chemokines in IBD? Inflamm Bowel Dis 14:S117–S118
Fuss IJ, Neurath M, Boirivant M et al (1996) Disparate CD4+ lamina propria (LP) lymphokine secretion profiles in inflammatory bowel disease. Crohn’s disease LP cells manifest increased secretion of IFN-γ, whereas ulcerative colitis LP cells manifest increased secretion of IL-5. J Immunol 157:1261–1270
Gerlach K, Hwang Y, Nikolaev A, Atreya R et al (2014) TH9 cells that express the transcription factor PU.1 drive T cell-mediated colitis via IL-9 receptor signaling in intestinal epithelial cells. Nat Immunol 15:676–686
Boirivant M, Fuss IJ, Chu A, Strober W (1998) Oxazolone colitis: a murine model of T helper cell type 2 colitis treatable with antibodies to interleukin 4. J Exp Med 188:1929–1939
Heller F, Fuss IJ, Nieuwenhuis EE, Blumberg RS, Strober W (2002) Oxazolone colitis, a Th2 colitis model resembling ulcerative colitis, is mediated by IL-13-producing NK-T cells. Immunity 17:629–638
Daniel C, Sartory NA, Zahn N, Schmidt R et al (2007) FTY720 ameliorates oxazolone colitis in mice by directly affecting T helper type 2 functions. Mol Immunol 44:3305–3316
Iijima H, Neurath MF, Nagaishi T, Glickman JN et al (2004) Specific regulation of T helper cell 1-mediated murine colitis by CEACAM1. J Exp Med 199:471–482
Nolte T, Zadeh-Khorasani M, Safarov O, Rueff F et al (2013) Oxazolone and ethanol induce colitis in non-obese diabetic-severe combined immunodeficiency interleukin-2Rγ(null) mice engrafted with human peripheral blood mononuclear cells. Clin Exp Immunol 172:349–362
Schiechl G, Bauer B, Fuss I, Lang SA et al (2011) Tumor development in murine ulcerative colitis depends on MyD88 signaling of colonic F4/80+ CD11b(high)Gr1(low) macrophages. J Clin Invest 121:1692–1708
Becker C, Fantini MC, Wirtz S, Nikolaev A et al (2005) In vivo imaging of colitis and colon cancer development in mice using high resolution chromoendoscopy. Gut 54:950–954
Acknowledgment
This work was supported by DFG grant WE4656/2-2 and SFB 1181-B02.
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Weigmann, B., Neurath, M.F. (2016). Oxazolone-Induced Colitis as a Model of Th2 Immune Responses in the Intestinal Mucosa. In: Ivanov, A. (eds) Gastrointestinal Physiology and Diseases. Methods in Molecular Biology, vol 1422. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3603-8_23
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DOI: https://doi.org/10.1007/978-1-4939-3603-8_23
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