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Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS

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Endocannabinoid Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1412))

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Abstract

LC-MS/MS is a powerful analytical technique that provides unequivocal identification and reliable quantification of the analytes, using Selected Reaction Monitoring or Multi Reaction Monitoring acquisition mode.

2-Arachidonoylglycerol (2-AG) is the most abundant endocannabinoid, which plays a major role in a wide variety of physiological and pathological processes. Analysis of 2-AG by means of LC-MS/MS allows the detection of very low concentrations in biological samples. Here, we describe how to determine 2-AG levels in tiny samples of tissues and plasma through LC-MS/MS, by using very quick and easy to perform extraction procedures, with reduced solvent consumption.

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Authors and Affiliations

  1. Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Via 1, Teramo, 64023, Italy

    Natalia Battista & Manuel Sergi

Authors
  1. Natalia Battista
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  2. Manuel Sergi
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Corresponding author

Correspondence to Manuel Sergi .

Editor information

Editors and Affiliations

  1. Campus Bio-Medico University of Rome, Department of Medicine, Rome, Italy

    Mauro Maccarrone

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Battista, N., Sergi, M. (2016). Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS. In: Maccarrone, M. (eds) Endocannabinoid Signaling. Methods in Molecular Biology, vol 1412. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3539-0_3

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  • DOI: https://doi.org/10.1007/978-1-4939-3539-0_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3537-6

  • Online ISBN: 978-1-4939-3539-0

  • eBook Packages: Springer Protocols

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