Skip to main content
SpringerLink
Log in

Micro-fractional Epidermal Powder Delivery for Skin Vaccination

  • Protocol
  • First Online:
Vaccine Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1404))

Abstract

Skin is a highly immunogenic site for vaccine delivery due to its richness of antigen-presenting cells. Several vaccines have been approved for skin delivery and in particular intradermal delivery in the last two decades. Yet intradermal delivery often causes frequent and severe local reactions, preventing the incorporation of adjuvants to further boost skin vaccination. Here we describe a novel skin delivery technology, called micro-fractional epidermal powder delivery or EPD, with minimized local reactions for improved skin vaccination. EPD is based on laser or microneedle treatment to generate microchannel arrays in the epidermis followed by topical application of powder vaccine-coated array patches to deliver vaccines into the skin via microchannels. Due to the fractional delivery, EPD significantly reduces vaccine/adjuvant-induced local reactions without compromising vaccine immunogenicity and adjuvant potency. EPD also eliminates needle injection-associated pain and is promising to improve vaccine stability due to the direct powder delivery. This chapter describes detailed methods for the advantageous EPD in preclinical animal models.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 249.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Kupper TS (2012) Old and new: recent innovations in vaccine biology and skin T cells. J Invest Dermatol 132(3 Pt 2):829–834

    Article  CAS  Google Scholar 

  2. Nicolas JF, Guy B (2008) Intradermal, epidermal and transcutaneous vaccination: from immunology to clinical practice. Expert Rev Vaccines 7:1201–1214

    Article  Google Scholar 

  3. Kim YC, Prausnitz MR (2011) Enabling skin vaccination using new delivery technologies. Drug Deliv Transl Res 1:7–12

    Article  Google Scholar 

  4. Lang J, Hoa DQ, Gioi NV et al (1999) Immunogenicity and safety of low-dose intradermal rabies vaccination given during an Expanded Programme on immunization session in Viet Nam: results of a comparative randomized trial. Trans R Soc Trop Med Hyg 93:208–213

    Article  CAS  Google Scholar 

  5. Beran J, Ambrozaitis A, Laiskonis A et al (2009) Intradermal influenza vaccination of healthy adults using a new microinjection system: a 3-year randomised controlled safety and immunogenicity trial. BMC Med 7:13

    Article  Google Scholar 

  6. Leroux-Roels I, Vets E, Freese R et al (2008) Seasonal influenza vaccine delivered by intradermal microinjection: a randomised controlled safety and immunogenicity trial in adults. Vaccine 26:6614–6619

    Article  CAS  Google Scholar 

  7. Leroux-Roels I, Weber F (2013) Intanza® 9 μg intradermal seasonal influenza vaccine for adults 18 to 59 years of age. Hum Vaccin Immunother 9:115–121

    Article  CAS  Google Scholar 

  8. Madhusudana SN, Mani RS (2014) Intradermal vaccination for rabies prophylaxis: conceptualization, evolution, present status and future. Expert Rev Vaccines 13:641–655

    Article  CAS  Google Scholar 

  9. Grubeck-Loebenstein B, Della BS, Iorio AM, Michel JP, Pawelec G, Solana R (2009) Immunosenescence and vaccine failure in the elderly. Aging Clin Exp Res 21:201–209

    Article  CAS  Google Scholar 

  10. Boraschi D, Italiani P (2014) Immunosenescence and vaccine failure in the elderly: strategies for improving response. Immunol Lett 162(1 Pt B):346–353

    Article  CAS  Google Scholar 

  11. Holland D, Booy R, De LF et al (2008) Intradermal influenza vaccine administered using a new microinjection system produces superior immunogenicity in elderly adults: a randomized controlled trial. J Infect Dis 198:650–658

    Article  Google Scholar 

  12. Chen X, Wu MX (2011) Laser vaccine adjuvant for cutaneous immunization. Expert Rev Vaccines 10:1397–1403

    Article  CAS  Google Scholar 

  13. Chen X, Wang J, Shah D, Wu MX (2013) An update on the use of laser technology in skin vaccination. Expert Rev Vaccines 12:1313–1323

    Article  CAS  Google Scholar 

  14. Krejci J, Nechvatalova K, Kudlackova H et al (2013) Effects of adjuvants on the immune response of pigs after intradermal administration of antigen. Res Vet Sci 94:73–76

    Article  CAS  Google Scholar 

  15. Ward JR, Jones RS (1962) Studies on adjuvant-induced polyarthritis in rats. I. Adjuvant composition, route of injection, and removal of depot site. Arthritis Rheum 5:557–564

    Article  CAS  Google Scholar 

  16. Chen X, Kositratna G, Zhou C, Manstein D, Wu MX (2014) Micro-fractional epidermal powder delivery for improved skin vaccination. J Control Release 192:310–316

    Article  CAS  Google Scholar 

  17. Chen X, Kim P, Farinelli B et al (2010) A novel laser vaccine adjuvant increases the motility of antigen presenting cells. PLoS One 5(10):e13776

    Article  Google Scholar 

Download references

Acknowledgement

This work is supported by the National Institutes of Health grants DA033371 and AI107678 (to X.Y.C.) and AI089779 and DA028378 (to M.X.W.).

Author information

Author notes

  1. Xinyuan Chen

    Present address: Wellman Center for Photomedicine, Massachusetts General Hospital, Department of Dermatology, Harvard Medical School, 50 Blossom Street, Edwards 222, Boston, MA, 02114, USA

Authors and Affiliations

  1. Wellman Center for Photomedicine, Massachusetts General Hospital, Department of Dermatology, Harvard Medical School, 50 Blossom Street, Edwards 222, Boston, MA, 02114, USA

    Feng Jia, Shengwu Liu & Mei X. Wu

  2. Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, 7 Greenhouse Road, Room 395L, Kingston, RI, 02881, USA

    Xinyuan Chen

Authors
  1. Feng Jia
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shengwu Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mei X. Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Xinyuan Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Xinyuan Chen .

Editor information

Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, PA, USA

    Sunil Thomas

Rights and permissions

Reprints and permissions

Copyright information

© 2016 Springer Science+Business Media New York

About this protocol

Cite this protocol

Jia, F., Liu, S., Wu, M.X., Chen, X. (2016). Micro-fractional Epidermal Powder Delivery for Skin Vaccination. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 1404. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-3389-1_46

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-3389-1_46

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-3388-4

  • Online ISBN: 978-1-4939-3389-1

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation