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Preparation and Characterization of PLGA Encapsulated Protective Antigen Domain 4 Nanoformulation

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Vaccine Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1404))

Abstract

Poly (lactic-co-glycolic acid) (PLGA) based particulate systems have been widely explored for the development of subunit based vaccines owing to its biodegradability, biocompatibility, controlled release of entrapped antigen, targeted delivery potential, and nontoxic degradation product. Here, we describe the preparation of PLGA encapsulated recombinant protective antigen domain 4 (PAD4) nanoformulation (PAD4-NP) and its characterization for antigen content, morphology, and size. We also discuss the method of immunization in mice models to evaluate such PLGA based nanoformulation vaccines.

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Acknowledgements

The work was supported in part by funding from the Department of Biotechnology (DBT) and Department of Science and Technology (DST), India. MM is a recipient of Fulbright Fellowship, Utrecht University short stay Fellowship, CSIR, SBRI-India GID Fellowship (NIH Research Grant number D43 TW000924 funded by the Fogarty International Center), and ICMR Fellowship. SS is a recipient of Ramalingaswami Fellowship of DBT, India.

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Authors and Affiliations

  1. Laboratory of Molecular Biology and Genetic Engineering, School of Biotechnology, Jawaharlal Nehru University, New Delhi, 110067, India

    Manish Manish & Rakesh Bhatnagar

  2. Department of Biosciences, Manipal University Jaipur, Jaipur-Ajmer Express Highway, Jaipur, Rajasthan, 303007, India

    Manish Manish

  3. Department of Microbial Biotechnology, Panjab University, Chandigarh, 160014, India

    Samer Singh

Authors
  1. Manish Manish
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  2. Rakesh Bhatnagar
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  3. Samer Singh
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Corresponding author

Correspondence to Samer Singh .

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Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, PA, USA

    Sunil Thomas

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Manish, M., Bhatnagar, R., Singh, S. (2016). Preparation and Characterization of PLGA Encapsulated Protective Antigen Domain 4 Nanoformulation. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 1404. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-3389-1_43

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  • DOI: https://doi.org/10.1007/978-1-4939-3389-1_43

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-3388-4

  • Online ISBN: 978-1-4939-3389-1

  • eBook Packages: Springer Protocols

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