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Development of Mycoplasma hyopneumoniae Recombinant Vaccines

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Vaccine Design

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1404))

Abstract

Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia (EP), a disease that affects swine production worldwide. Vaccination is the most cost-effective strategy for the control and prevention of the disease. Research using genome-based approach has the potential to elucidate the biology and pathogenesis of M. hyopneumoniae and contribute to the development of more effective vaccines. Here, we describe the protocol for developing M. hyopneumoniae recombinant vaccines using reverse vaccinology approaches.

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References

  1. Thacker EL, Minion FC (2010) Mycoplasmosis. In: Zimmerman J (ed) Diseases of swine. Iowa State University Press, Ames, Iowa, USA, pp 779–797

    Google Scholar 

  2. Maes D, Segales J, Meyns T et al (2008) Control of Mycoplasma hyopneumoniae infections in pigs. Vet Microbiol 126:297–309

    Article  CAS  Google Scholar 

  3. Vicca J, Stakenborg T, Maes D et al (2004) In vitro susceptibilities of Mycoplasma hyopneumoniae field isolates. Antimicrob Agents Chemother 48:4470–4472

    Article  CAS  Google Scholar 

  4. Haesebrouck F, Pasmans F, Chiers K et al (2004) Efficacy of vaccines against bacterial diseases in swine: what can we expect? Vet Microbiol 100:255–268

    Article  CAS  Google Scholar 

  5. Rappuoli R (2001) Reverse vaccinology, a genome-based approach to vaccine development. Vaccine 19:2688–2691

    Article  CAS  Google Scholar 

  6. Razin S, Yogev D, Naot Y (1998) Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev 62:1094–1156

    Article  CAS  Google Scholar 

  7. Nuc P, Nuc K (2006) Recombinant protein production in Escherichia coli. Postepy Biochemii 52:448–456

    CAS  PubMed  Google Scholar 

  8. Simionatto S, Marchioro SB, Galli V et al (2009) Efficient site-directed mutagenesis using an overlap extension-PCR method for expressing Mycoplasma hyopneumoniae genes in Escherichia coli. J Microbiol Methods 79:101–105

    Article  CAS  Google Scholar 

  9. Galli V, Simionatto S, Marchioro SB et al (2012) Immunization of mice with Mycoplasma hyopneumoniae antigens P37, P42, P46 and P95 delivered as recombinant subunit or DNA vaccines. Vaccine 31:135–140

    Article  CAS  Google Scholar 

  10. Simionatto S, Marchioro SB, Galli V et al (2012) Immunological characterization of Mycoplasma hyopneumoniae recombinant proteins. Comp Immunol Microb 35:209–216

    Article  Google Scholar 

  11. Chen AY, Fry SR, Forbes-Faulkner J et al (2006) Evaluation of the immunogenicity of the P97R1 adhesin of Mycoplasma hyopneumoniae as a mucosal vaccine in mice. J Med Microbiol 55:923–929

    Article  CAS  Google Scholar 

  12. Chen AY, Fry SR, Forbes-Faulkner J et al (2006) Comparative immunogenicity of M. hyopneumoniae NrdF encoded in different expression systems delivered orally via attenuated S. typhimurium aroA in mice. Vet Microbiol 114:252–259

    Article  CAS  Google Scholar 

  13. Chen JR, Liao CW, Mao SJ et al (2001) A recombinant chimera composed of repeat region RR1 of Mycoplasma hyopneumoniae adhesin with Pseudomonas exotoxin: in vivo evaluation of specific IgG response in mice and pigs. Vet Microbiol 80:347–357

    Article  CAS  Google Scholar 

  14. Fagan PK, Walker MJ, Chin J et al (2001) Oral immunization of swine with attenuated Salmonella typhimurium aroA SL3261 expressing a recombinant antigen of Mycoplasma hyopneumoniae (NrdF) primes the immune system for a NrdF specific secretory IgA response in the lungs. Microb Pathog 30:101–110

    Article  CAS  Google Scholar 

  15. Okamba FR, Arella M, Music N et al (2010) Potential use of a recombinant replication-defective adenovirus vector carrying the C-terminal portion of the P97 adhesin protein as a vaccine against Mycoplasma hyopneumoniae in swine. Vaccine 28:4802–4809

    Article  CAS  Google Scholar 

  16. Okamba FR, Moreau E, Cheikh Saad BK et al (2007) Immune responses induced by replication-defective adenovirus expressing the C-terminal portion of the Mycoplasma hyopneumoniae P97 adhesin. Clin Vaccine Immunol 14:767–774

    Article  CAS  Google Scholar 

  17. Shimoji Y, Oishi E, Muneta Y et al (2003) Vaccine efficacy of the attenuated Erysipelothrix rhusiopathiae YS-19 expressing a recombinant protein of Mycoplasma hyopneumoniae P97 adhesin against mycoplasmal pneumonia of swine. Vaccine 21:532–537

    Article  CAS  Google Scholar 

  18. Zou HY, Liu XJ, Ma FY et al (2011) Attenuated Actinobacillus pleuropneumoniae as a bacterial vector for expression of Mycoplasma hyopneumoniae P36 gene. J Gene Med 13:221–229

    Article  CAS  Google Scholar 

  19. Conceicao FR, Moreira AN, Dellagostin OA (2006) A recombinant chimera composed of R1 repeat region of Mycoplasma hyopneumoniae P97 adhesin with Escherichia coli heat-labile enterotoxin B subunit elicits immune response in mice. Vaccine 24:5734–5743

    Article  CAS  Google Scholar 

  20. Marchioro SB, Sácristan Rdel P, Michiels A et al (2014) Immune responses of a chimaeric protein vaccine containing Mycoplasma hyopneumoniae antigens and LTB against experimental M. hyopneumoniae infection in pigs. Vaccine 32:4689–4694

    Article  CAS  Google Scholar 

  21. Chen AY, Fry SR, Daggard GE et al (2008) Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice. Vaccine 26:4372–4378

    Article  CAS  Google Scholar 

  22. Galli V, Simionatto S, Marchioro SB et al (2013) Recombinant secreted antigens from Mycoplasma hyopneumoniae delivered as a cocktail vaccine enhance the immune response of mice. Clin Vaccine Immunol 20:1370–1376

    Article  CAS  Google Scholar 

  23. Pinto PM, Chemale G, de Castro LA et al (2007) Proteomic survey of the pathogenic Mycoplasma hyopneumoniae strain 7448 and identification of novel post-translationally modified and antigenic proteins. Vet Microbiol 121:83–93

    Article  CAS  Google Scholar 

  24. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

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Acknowledgements

This chapter described work supported by Fundação de Amparo a Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazil.

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Authors and Affiliations

  1. Faculdade de Ciências da Saúde, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 533, Dourados, MS, CEP 79804-970, Brazil

    Silvana Beutinger Marchioro

  2. Faculdade de Ciências Biológicas e Ambientais, Universidade Federal da Grande Dourados, Rodovia Dourados – Itahum, Km 12 – Cidade Universitaria, Cx. Postal 533, Dourados, MS, CEP 79804-970, Brazil

    Simone Simionatto

  3. Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Campus Universitário s/n, Prédio 19, Caixa Postal 354, Pelotas, RS, 96010-900, Brazil

    Odir Dellagostin

Authors
  1. Silvana Beutinger Marchioro
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  2. Simone Simionatto
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  3. Odir Dellagostin
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Corresponding author

Correspondence to Odir Dellagostin .

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Editors and Affiliations

  1. Lankenau Institute for Medical Research, Wynnewood, PA, USA

    Sunil Thomas

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Marchioro, S.B., Simionatto, S., Dellagostin, O. (2016). Development of Mycoplasma hyopneumoniae Recombinant Vaccines. In: Thomas, S. (eds) Vaccine Design. Methods in Molecular Biology, vol 1404. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-3389-1_2

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  • DOI: https://doi.org/10.1007/978-1-4939-3389-1_2

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-3388-4

  • Online ISBN: 978-1-4939-3389-1

  • eBook Packages: Springer Protocols

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