Abstract
Secreted luciferases provide a simple, accurate, and cost-effective tool to monitor tumor response after treatment in small animal models. This protocol describes all the steps required to generate human tumor cell lines expressing this reporter gene and to monitor in vivo tumor progression after injection in immunocompromised mice by means of serial peripheral blood sampling and analysis.
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© 2016 Springer Science+Business Media New York
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Falcone, L., Casucci, M. (2016). Exploiting Secreted Luciferases to Monitor Tumor Progression In Vivo. In: Bondanza, A., Casucci, M. (eds) Tumor Immunology. Methods in Molecular Biology, vol 1393. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3338-9_10
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DOI: https://doi.org/10.1007/978-1-4939-3338-9_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3336-5
Online ISBN: 978-1-4939-3338-9
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