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Posttranscriptional Regulation of PTEN by Competing Endogenous RNAs

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PTEN

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1388))

Abstract

PTEN expression can be dysregulated in cancers via multiple mechanisms including genomic loss, epigenetic silencing, transcriptional repression, and posttranscriptional regulation by microRNAs. MicroRNAs are short, noncoding RNAs that regulate gene expression by binding to recognition sites on target transcripts. Recent studies have demonstrated that the competition for shared microRNAs between both protein-coding and noncoding transcripts represents an additional facet of gene regulation. Here, we describe in detail an integrated computational and experimental approach to identify and validate these competing endogenous RNA (ceRNA) interactions.

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Acknowledgements

We thank Shen Mynn Tan for critical reading of the manuscript. Y.T. was supported by a Singapore National Research Foundation Fellowship and a National University of Singapore President’s Assistant Professorship. P.P.P. was supported in part by NIH grants 1R01CA170158 and R01 CA82328.

Author information

Authors and Affiliations

  1. Cancer Science Institute of Singapore, Centre for Translational Medicine, National University of Singapore, 14 Medical Drive, #12-01, Singapore, 117599, Singapore

    Yvonne Tay Ph.D.

  2. Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, 117597, Singapore

    Yvonne Tay Ph.D.

  3. Cancer Research Institute, Beth Israel Deaconess Cancer Center, Department of Medicine and Pathology, Beth Israel Deaconess Medical Center, Harvard Medical School, 3 Blackfan Circle, Boston, MA, 02215, USA

    Pier Paolo Pandolfi M.D., Ph.D.

Authors
  1. Yvonne Tay Ph.D.
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  2. Pier Paolo Pandolfi M.D., Ph.D.
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Corresponding authors

Correspondence to Yvonne Tay Ph.D. or Pier Paolo Pandolfi M.D., Ph.D. .

Editor information

Editors and Affiliations

  1. University of Toronto, Princess Margaret Cancer Centre, Toronto, Ontario, Canada

    Leonardo Salmena

  2. University of Toronto, Princess Margaret Cancer Centre, Toronto, Ontario, Canada

    Vuk Stambolic

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Tay, Y., Pandolfi, P.P. (2016). Posttranscriptional Regulation of PTEN by Competing Endogenous RNAs. In: Salmena, L., Stambolic, V. (eds) PTEN. Methods in Molecular Biology, vol 1388. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3299-3_10

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  • DOI: https://doi.org/10.1007/978-1-4939-3299-3_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3297-9

  • Online ISBN: 978-1-4939-3299-3

  • eBook Packages: Springer Protocols

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