Abstract
Electroporation has been widely used to efficiently transfer foreign genes into the mammalian central nervous system (CNS), and thus plays an important role in gene therapeutic studies on some brain disorders. A lot of work concerning electroporation is focused on gene transfer into rodent brains. This technique involves an injection of nucleic acids into the brain ventricle or specific area and then applying appropriate electrical field to the injected area. Here, we briefly introduced the advantages and the basic procedures of gene transfer into the rodent brain using electroporation. Better understanding of electroporation in rodent brain may further facilitate gene therapeutic studies on brain disorders.
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Acknowledgement
This work was supported by the Natural Science Foundation of China (31200822), the grants of Beijing Natural Science Foundation (5122033), and the National Basic Research Programs of China (grant number 2012CB518200).
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Ding, XF., Fan, M. (2016). Nonviral Gene Therapy of the Nervous System: Electroporation. In: Manfredsson, F. (eds) Gene Therapy for Neurological Disorders. Methods in Molecular Biology, vol 1382. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3271-9_22
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DOI: https://doi.org/10.1007/978-1-4939-3271-9_22
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3270-2
Online ISBN: 978-1-4939-3271-9
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