Abstract
The enteric nervous system (ENS) is a complex network of ganglia embedded in the walls of the gastrointestinal (GI) tract. The highly integrated enteric neural network can act independently of sympathetic and parasympathetic innervation to control all aspects of GI function. Due to the central role the ENS plays in GI physiology, the ability to manipulate genetic expression within the ENS is an invaluable tool for both research and clinical applications. Here, we describe a method for gene delivery to the ENS using direct injections of adeno-associated virus (AAV) into the gut wall. This method is able to achieve transgene expression in both neurons and glia located in discrete areas of the ENS.
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References
Wood JD (2011) Enteric nervous system: the-brain-in-the-gut. Morgan & Claypool Life Sciences, New Jersey
Belkind-Gerson J, Graeme-Cook F, Winter H (2006) Enteric nervous system disease and recovery, plasticity, and regeneration. J Pediatr Gastroenterol Nutr 42:343–350
During MJ et al (2000) An oral vaccine against NMDAR1 with efficacy in experimental stroke and epilepsy. Science 287:1453–1460
During MJ et al (1998) Peroral gene therapy of lactose intolerance using an adeno-associated virus vector. Nat Med 4:1131–1135
Foust KD et al (2009) Intravascular AAV9 preferentially targets neonatal neurons and adult astrocytes. Nat Biotechnol 27:59–65
Zincarelli C, Soltys S, Rengo G, Rabinowitz JE (2008) Analysis of AAV serotypes 1-9 mediated gene expression and tropism in mice after systemic injection. Mol Ther 16:1073–1080
Benskey MJ et al (2015) Targeted gene delivery to the enteric nervous system using AAV: a comparison across serotypes and capsid mutants. Mol Ther. doi:10.1038/mt.2015.7
Acknowledgement
This work was supported by a Strategic Partnership Grant from the Michigan State University Foundation.
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© 2016 Springer Science+Business Media New York
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Benskey, M.J., Manfredsson, F.P. (2016). Gene Therapy of the Peripheral Nervous System: The Enteric Nervous System. In: Manfredsson, F. (eds) Gene Therapy for Neurological Disorders. Methods in Molecular Biology, vol 1382. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3271-9_19
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DOI: https://doi.org/10.1007/978-1-4939-3271-9_19
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3270-2
Online ISBN: 978-1-4939-3271-9
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