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Quantitative DNA Methylation Profiling in Cancer

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Cancer Gene Profiling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1381))

Abstract

Epigenetic mechanisms including DNA methylation are fundamental for the regulation of gene expression. Epigenetic alterations can lead to the development and the evolution of malignant tumors as well as the emergence of phenotypically different cancer cells or metastasis from one single tumor cell. Here we describe bisulfite pyrosequencing, a technology to perform quantitative DNA methylation analyses, to detect aberrant DNA methylation in malignant tumors.

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Author information

Authors and Affiliations

  1. Institute of Human Genetics, University of Kiel and University Hospital Schleswig-Holstein, Campus Kiel, Schwanenweg 24, Kiel, 24105, Germany

    Ole Ammerpohl Ph.D., Andrea Haake, Julia Kolarova & Reiner Siebert

Authors
  1. Ole Ammerpohl Ph.D.
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  2. Andrea Haake
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  3. Julia Kolarova
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  4. Reiner Siebert
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Corresponding author

Correspondence to Ole Ammerpohl Ph.D. .

Editor information

Editors and Affiliations

  1. Department of Surgery, Universitätsklinikum Erlangen, Erlangen, Germany

    Robert Grützmann

  2. Department of Surgery, TU-Dresden, Dresden, Germany

    Christian Pilarsky

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Ammerpohl, O., Haake, A., Kolarova, J., Siebert, R. (2016). Quantitative DNA Methylation Profiling in Cancer. In: Grützmann, R., Pilarsky, C. (eds) Cancer Gene Profiling. Methods in Molecular Biology, vol 1381. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3204-7_5

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  • DOI: https://doi.org/10.1007/978-1-4939-3204-7_5

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3203-0

  • Online ISBN: 978-1-4939-3204-7

  • eBook Packages: Springer Protocols

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