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Detection of Phosphorylation Status of Cytokinetic Components

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Yeast Cytokinesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1369))

Abstract

Yeast cells can be easily cultured, synchronized, and genetically modified making them a convenient model system to study molecular mechanisms underlying cytokinesis. Here, we describe simple methods that allow the analysis of the phosphorylation profile of cytokinetic proteins, both in vivo and in vitro, using standard laboratory equipment. In addition, we compare the ability of three different, standard, and optimized acrylamide gel conditions to separate phosphorylated forms, using the protein Inn1 as an example.

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Author information

Authors and Affiliations

  1. DKFZ-ZMBH Alliance, German Cancer Research Center (DKFZ), University of Heidelberg, Im Neuenheimer Feld 581, Heidelberg, 69120, Germany

    Franz Meitinger & Gislene Pereira

  2. Ludwig Institute for Cancer Research, 9500 Gilman Drive, CMM East, La Jolla, CA, 92093, USA

    Franz Meitinger

  3. Division of Biomedical Cell Biology, Warwick Medical School, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, UK

    Saravanan Palani

Authors
  1. Franz Meitinger
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  2. Saravanan Palani
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  3. Gislene Pereira
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Corresponding author

Correspondence to Gislene Pereira .

Editor information

Editors and Affiliations

  1. Departamento de Biología Molecular, Universidad de Cantabria, Facultad de Medicina, Cardenal Herrera Oria s/n, Instituto de Biomedicina y Biotecnología de Cantabria, Universidad de Cantabria, CSIC, Santander, Spain

    Alberto Sanchez-Diaz

  2. Universidad de Salamanca, Instituto de Biología Funcional y Genómica, CSIC, Salamanca, Spain

    Pilar Perez

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Meitinger, F., Palani, S., Pereira, G. (2016). Detection of Phosphorylation Status of Cytokinetic Components. In: Sanchez-Diaz, A., Perez, P. (eds) Yeast Cytokinesis. Methods in Molecular Biology, vol 1369. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3145-3_16

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  • DOI: https://doi.org/10.1007/978-1-4939-3145-3_16

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3144-6

  • Online ISBN: 978-1-4939-3145-3

  • eBook Packages: Springer Protocols

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