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Introduction to Proteomics Technologies

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Statistical Analysis in Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1362))

Abstract

Compared to genomics or transcriptomics, proteomics is often regarded as an “emerging technology,” i.e., as not having reached the same level of maturity. While the successful implementation of proteomics workflows and technology still requires significant levels of expertise and specialization, great strides have been made to make the technology more powerful, streamlined and accessible. In 2014, two landmark studies published the first draft versions of the human proteome.

We aim to provide an introduction specifically into the background of mass spectrometry (MS)-based proteomics. Within the field, mass spectrometry has emerged as a core technology. Coupled to increasingly powerful separations and data processing and bioinformatics solution, it allows the quantitative analysis of whole proteomes within a matter of days, a timescale that has made global comparative proteome studies feasible at last. We present and discuss the basic concepts behind proteomics mass spectrometry and the accompanying topic of protein and peptide separations, with a focus on the properties of datasets emerging from such studies.

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Authors and Affiliations

  1. Bioanalytical Mass Spectrometry, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany

    Christof Lenz

  2. Core Facility Proteomics, Institute of Clinical Chemistry, University Medical Center, Göttingen, Germany

    Christof Lenz

  3. Clinic of Nephrology and Rheumatology, University Medical Center, Robert-Koch-Straße 40, 37075, Göttingen, Germany

    Hassan Dihazi

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  1. Christof Lenz
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  2. Hassan Dihazi
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Correspondence to Hassan Dihazi .

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Editors and Affiliations

  1. Department of Medical Statistics, University Medical Center Göttingen, Göttingen, Germany

    Klaus Jung

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Lenz, C., Dihazi, H. (2016). Introduction to Proteomics Technologies. In: Jung, K. (eds) Statistical Analysis in Proteomics. Methods in Molecular Biology, vol 1362. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3106-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-3106-4_1

  • Publisher Name: Humana Press, New York, NY

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