Abstract
G protein-coupled receptors (GPCRs) constitute the largest family of plasma membrane receptors, thus representing the more investigated drug targets in the design of new therapeutic strategies. In this family of receptors, the binding of an agonist typically triggers the activation of heterotrimeric G proteins, which in turn control the propagation of secondary messenger molecules, such as cAMP, which play a key role in important physiological processes. Accordingly, determining GPCR-mediated cAMP accumulation in native tissue (i.e., synaptosomes) constitutes an important step in the pharmacological characterization of these receptors. Here, we describe the methodology used to assess GPCR-mediated cAMP accumulation in rat striatal synaptosomes.
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Acknowledgements
This work was supported by Ministerio de Economía y Competitividad/Instituto de Salud Carlos III (SAF2014-55700-P, PCIN-2013-019-C03-03, and PIE14/00034), Institució Catalana de Recerca i Estudis Avançats (ICREA Academia-2010), and Agentschap voor Innovatie door Wetenschap en Technologie (SBO-140028) to FC. Also, F.C., J.T., and V.F.-D. belong to the “Neuropharmacology and Pain” accredited research group (Generalitat de Catalunya, 2014 SGR 1251). We thank E. Castaño and B. Torrejón from the Scientific and Technical Services (SCT) group at the Bellvitge Campus of the University of Barcelona for their technical assistance.
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Taura, J., Fernández-Dueñas, V., Ciruela, F. (2016). Determination of GPCR-Mediated cAMP Accumulation in Rat Striatal Synaptosomes. In: Luján, R., Ciruela, F. (eds) Receptor and Ion Channel Detection in the Brain. Neuromethods, vol 110. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3064-7_28
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DOI: https://doi.org/10.1007/978-1-4939-3064-7_28
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3063-0
Online ISBN: 978-1-4939-3064-7
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