Abstract
Anther culture is the most popular of the techniques used to induce microspore embryogenesis. This technique is well set up in a wide range of crops, including pepper. In this chapter, a protocol for anther culture in pepper is described. The protocol presented hereby includes the steps from the selection of buds from donor plants to the regeneration and acclimatization of doubled haploid plants derived from the embryos, as well as a description of how to analyze the ploidy level of the regenerated plants.
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Acknowledgments
This work was supported by the grant AGL2014-55177 from Spanish MINECO to J.M.S.S.
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Parra-Vega, V., Seguí-Simarro, J.M. (2016). Anther Culture in Pepper (Capsicum annuum L.). In: Germana, M., Lambardi, M. (eds) In Vitro Embryogenesis in Higher Plants. Methods in Molecular Biology, vol 1359. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3061-6_26
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DOI: https://doi.org/10.1007/978-1-4939-3061-6_26
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-3060-9
Online ISBN: 978-1-4939-3061-6
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