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Chromatin Immunoprecipitation (ChIP) Assay in Candida albicans

  • Protocol
Candida Species

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1356))

Abstract

Chromatin immunoprecipitation (ChIP) is a widely used technique which can determine the in vivo association of a specific protein on a particular DNA locus in the genome. In this method cross-linked chromatin is sheared and immunoprecipitated with antibodies raised against a target protein of interest. The end result of this process is the enrichment of DNA fragments associated with the desired protein. Thus, interactions between proteins and genomic loci in cellular context can be determined by this technique. Here, we are describing a ChIP protocol that is optimized for Candida albicans. The protocol requires 4–5 days for completion of the assay and has been used to produce robust ChIP results for diverse proteins in this organism and its related species including Candida dubliniensis and Candida tropicalis.

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Acknowledgements

The ChIP method for Candida albicans was first developed by KS in John Carbon’s laboratory and further modified. The work was supported by a grant from the Department of Biotechnology, Government of India, to KS. The intramural support from JNCASR is also acknowledged. SM, LSR, and GC were supported by senior research fellowships from Council of Scientific and Industrial Research (CSIR), Government of India.

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Authors and Affiliations

  1. Molecular Mycology Laboratory, Molecular Biology and Genetics Unit, Jawaharlal Nehru Centre for Advanced Scientific Research, Jakkur, Bangalore, 560064, India

    Sreyoshi Mitra, Laxmi Shanker Rai, Gautam Chatterjee & Kaustuv Sanyal

Authors
  1. Sreyoshi Mitra
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  2. Laxmi Shanker Rai
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  3. Gautam Chatterjee
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  4. Kaustuv Sanyal
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Corresponding author

Correspondence to Kaustuv Sanyal .

Editor information

Editors and Affiliations

  1. Dept Microbiology & Immunology, Georgetown University Medical Center, Washington, District of Columbia, USA

    Richard Calderone

  2. Dept of Microbiology & Immunology, Georgetown University Medical Center, Washington, District of Columbia, USA

    Ronald Cihlar

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Mitra, S., Rai, L.S., Chatterjee, G., Sanyal, K. (2016). Chromatin Immunoprecipitation (ChIP) Assay in Candida albicans . In: Calderone, R., Cihlar, R. (eds) Candida Species. Methods in Molecular Biology, vol 1356. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3052-4_4

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  • DOI: https://doi.org/10.1007/978-1-4939-3052-4_4

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3051-7

  • Online ISBN: 978-1-4939-3052-4

  • eBook Packages: Springer Protocols

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