Abstract
The aim of this chapter is to give a short introduction to peptide analysis by mass spectrometry (MS) and interpretation of fragment mass spectra. Through examples and guidelines we demonstrate how to understand and validate search results and how to perform de novo sequencing based on the often very complex fragmentation pattern obtained by tandem mass spectrometry (also referred to as MSMS). The focus is on simple rules for interpretation of MSMS spectra of tryptic as well as non-tryptic peptides.
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Anne-Katrine Vestergaard is acknowledged for excellent technical assistance.
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Hjernø, K., Højrup, P. (2015). Interpretation of Tandem Mass Spectrometry (MSMS) Spectra for Peptide Analysis. In: Houen, G. (eds) Peptide Antibodies. Methods in Molecular Biology, vol 1348. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2999-3_10
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DOI: https://doi.org/10.1007/978-1-4939-2999-3_10
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