Abstract
Smad7 is an important negative modulator that regulates the duration and strength of TGF-β signaling. Dysregulation of Smad7 is associated with the pathogenesis of many human diseases. Various mouse models are developed to facilitate addressing the physiological functions of Smad7. We have recently demonstrated that loss of Smad7 function by deletion in its MH2 domain leads to multiple cardiac defects and aggravates ethanol-induced liver injury. Here, we describe the procedure to construct and characterize the Smad7 conditional knockout mice.
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Acknowledgements
This work was supported by research grants from National Natural Science Foundation of China (81021002 and 81130077 to Y.C. and 30971660 to Y.P.), and Ministry of Science and Technology of China (2012CB524900 to Y.C. and 2010CB529506 to Y.P. and Z.W.).
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Pan, Y., Chen, Y. (2016). Generation and Characterization of Smad7 Conditional Knockout Mice. In: Feng, XH., Xu, P., Lin, X. (eds) TGF-β Signaling. Methods in Molecular Biology, vol 1344. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-2966-5_14
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DOI: https://doi.org/10.1007/978-1-4939-2966-5_14
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-2965-8
Online ISBN: 978-1-4939-2966-5
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